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-Structure paper
Title | Seeing the portal in herpes simplex virus type 1 B capsids. |
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Journal, issue, pages | J Virol, Vol. 85, Issue 4, Page 1871-1874, Year 2011 |
Publish date | Nov 24, 2010 |
Authors | R H Rochat / X Liu / K Murata / K Nagayama / F J Rixon / W Chiu / |
PubMed Abstract | Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron ...Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal. |
External links | J Virol / PubMed:21106752 / PubMed Central |
Methods | EM (single particle) |
Resolution | 24.0 Å |
Structure data | EMDB-5255: EMDB-5259: EMDB-5260: EMDB-5261: |