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TitleWaves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast.
Journal, issue, pagesDev Cell, Vol. 59, Issue 13, Page 1764-11782.e8, Year 2024
Publish dateJul 8, 2024
AuthorsRahel Wettstein / Jannik Hugener / Ludovic Gillet / Yi Hernández-Armenta / Adrian Henggeler / Jingwei Xu / Julian van Gerwen / Florian Wollweber / Meret Arter / Ruedi Aebersold / Pedro Beltrao / Martin Pilhofer / Joao Matos /
PubMed AbstractSexually reproducing eukaryotes employ a developmentally regulated cell division program-meiosis-to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a ...Sexually reproducing eukaryotes employ a developmentally regulated cell division program-meiosis-to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a proteomic census encompassing the entire meiotic program of budding yeast. We found that concerted waves of protein expression and phosphorylation modify nearly all cellular pathways to support meiotic entry, meiotic progression, and gamete morphogenesis. Leveraging this comprehensive resource, we pinpointed dynamic changes in mitochondrial components and showed that phosphorylation of the FF-ATP synthase complex is required for efficient gametogenesis. Furthermore, using cryoET as an orthogonal approach to visualize mitochondria, we uncovered highly ordered filament arrays of Ald4, a conserved aldehyde dehydrogenase that is highly expressed and phosphorylated during meiosis. Notably, phosphorylation-resistant mutants failed to accumulate filaments, suggesting that phosphorylation regulates context-specific Ald4 polymerization. Overall, this proteomic census constitutes a broad resource to guide the exploration of the unique sequence of events underpinning gametogenesis.
External linksDev Cell / PubMed:38906138
MethodsEM (tomography)
Structure data

EMDB-50229: Cryo-tomogram of FIB-milled vegetatively growing yeast cell with mitochondria
Method: EM (tomography)

EMDB-50230: Cryo-tomogram of FIB-milled pre-meiotic yeast cell with mitochondria
Method: EM (tomography)

EMDB-50231: Cryo-tomogram of FIB-milled meiotic yeast cell containing mitochondria with filaments
Method: EM (tomography)

EMDB-50232: Cryo-tomogram of FIB-milled yeast spore with mitochondria
Method: EM (tomography)

EMDB-50233: Cryo-tomogram of FIB-milled meiotic yeast cell containing mitochondria with filament arrays
Method: EM (tomography)

EMDB-50234: Cryo-tomogram of purified meiotic yeast mitochondria with Ald4 filaments
Method: EM (tomography)

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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