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TitleStructural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 2865, Year 2019
Publish dateJun 28, 2019
AuthorsJorge Cuéllar / W Grant Ludlam / Nicole C Tensmeyer / Takuma Aoba / Madhura Dhavale / César Santiago / M Teresa Bueno-Carrasco / Michael J Mann / Rebecca L Plimpton / Aman Makaju / Sarah Franklin / Barry M Willardson / José M Valpuesta /
PubMed AbstractThe mechanistic target of rapamycin (mTOR) kinase forms two multi-protein signaling complexes, mTORC1 and mTORC2, which are master regulators of cell growth, metabolism, survival and autophagy. Two ...The mechanistic target of rapamycin (mTOR) kinase forms two multi-protein signaling complexes, mTORC1 and mTORC2, which are master regulators of cell growth, metabolism, survival and autophagy. Two of the subunits of these complexes are mLST8 and Raptor, β-propeller proteins that stabilize the mTOR kinase and recruit substrates, respectively. Here we report that the eukaryotic chaperonin CCT plays a key role in mTORC assembly and signaling by folding both mLST8 and Raptor. A high resolution (4.0 Å) cryo-EM structure of the human mLST8-CCT intermediate isolated directly from cells shows mLST8 in a near-native state bound to CCT deep within the folding chamber between the two CCT rings, and interacting mainly with the disordered N- and C-termini of specific CCT subunits of both rings. These findings describe a unique function of CCT in mTORC assembly and a distinct binding site in CCT for mLST8, far from those found for similar β-propeller proteins.
External linksNat Commun / PubMed:31253771 / PubMed Central
MethodsEM (single particle)
Resolution3.97 - 7.5 Å
Structure data

4489

6qb8

EMDB-4489, PDB-6qb8:
Human CCT:mLST8 complex
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-4503:
Human substrate-free CCT
Method: EM (single particle) / Resolution: 7.5 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
KeywordsCHAPERONE / CCT / folding / WD40 / mLST8

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