Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.
Journal, issue, pages	Cell, Vol. 187, Issue 9, Page 2236-2249.e17, Year 2024
Publish date	Apr 25, 2024
Authors	Xian Xia / Po-Yu Sung / Michael W Martynowycz / Tamir Gonen / Polly Roy / Z Hong Zhou /
PubMed Abstract	Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques ...Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 Å, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond.
External links	Cell / PubMed:38614100 / PubMed Central
Methods	EM (single particle) / EM (subtomogram averaging)
Resolution	2.8 - 7.2 Å
Structure data	43714 8w12 EMDB-43714, PDB-8w12: Cryo-EM structure of VP3-VP6 heterohexamer Method: EM (single particle) / Resolution: 3.5 Å 43716 8w19 EMDB-43716, PDB-8w19: Cryo-EM structure of BTV star-subcore Method: EM (single particle) / Resolution: 4.4 Å 43719 8w1c EMDB-43719, PDB-8w1c: Cryo-EM structure of BTV pre-subcore Method: EM (single particle) / Resolution: 3.6 Å EMDB-43722: Cryo-EM structure of pre-subcore from in vitro assembled particles Method: EM (single particle) / Resolution: 3.5 Å EMDB-43723: Cryo-EM structure of BTV empty virion Method: EM (single particle) / Resolution: 2.9 Å EMDB-43724: Cryo-EM structure of BTV empty core Method: EM (single particle) / Resolution: 3.5 Å EMDB-43725: Cryo-EM structure of BTV empty pre-core Method: EM (single particle) / Resolution: 3.2 Å 43726 8w1i EMDB-43726, PDB-8w1i: Cryo-EM structure of BTV subcore Method: EM (single particle) / Resolution: 6.5 Å 43727 8w1o EMDB-43727, PDB-8w1o: Cryo-EM structure of BTV virion Method: EM (single particle) / Resolution: 2.8 Å EMDB-43728: Subtomogram averaging of BTV virion in host cells Method: EM (subtomogram averaging) / Resolution: 7.2 Å 43730 8w1r EMDB-43730, PDB-8w1r: Cryo-EM structure of BTV core Method: EM (single particle) / Resolution: 3.3 Å 43731 8w1s EMDB-43731, PDB-8w1s: Cryo-EM structure of BTV pre-core Method: EM (single particle) / Resolution: 3.1 Å
Source	bluetongue virus (serotype 1 / isolate south africa)
Keywords	VIRAL PROTEIN / Bluetongue virus / capsid protein / heterohexamer / assembly intermediate / pre-subcore / virion / core