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Title | Square beams for optimal tiling in transmission electron microscopy. |
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Journal, issue, pages | Nat Methods, Vol. 21, Issue 4, Page 562-565, Year 2024 |
Publish date | Jan 18, 2024 |
Authors | Eugene Y D Chua / Lambertus M Alink / Mykhailo Kopylov / Jake D Johnston / Fabian Eisenstein / Alex de Marco / |
PubMed Abstract | Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect ...Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem for dose-sensitive samples. Here, we introduce a square electron beam that can easily be retrofitted in existing microscopes, and demonstrate its application, showing that it can tile nearly perfectly and deliver cryo-electron microscopy imaging with a resolution comparable to conventional set-ups. |
External links | Nat Methods / PubMed:38238558 / PubMed Central |
Methods | EM (single particle) / EM (tomography) |
Resolution | 2.06 - 2.32 Å |
Structure data | EMDB-42371: Mouse apoferritin imaged with a square aperture EMDB-42372: Mouse apoferritin imaged with a round aperture EMDB-42373: Mouse apoferritin imaged with a square aperture with P2 projection lens rotation, reconstructed from 25,000 particles EMDB-42374: Mouse apoferritin imaged with a round aperture without P2 projection lens rotation, reconstructed from 25,000 particles EMDB-42851: 5x5 tiled montage tomogram of a holey carbon grid with apoferritin imaged with a square electron beam EMDB-42879: 3x3 tiled montage tomogram of a yeast lamella imaged with a square electron beam |
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