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TitleStructures of co-transcriptional RNA capping enzymes on paused transcription complex.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 4622, Year 2024
Publish dateMay 30, 2024
AuthorsYan Li / Qianmin Wang / Yanhui Xu / Ze Li /
PubMed AbstractThe 5'-end capping of nascent pre-mRNA represents the initial step in RNA processing, with evidence demonstrating that guanosine addition and 2'-O-ribose methylation occur in tandem with early steps ...The 5'-end capping of nascent pre-mRNA represents the initial step in RNA processing, with evidence demonstrating that guanosine addition and 2'-O-ribose methylation occur in tandem with early steps of transcription by RNA polymerase II, especially at the pausing stage. Here, we determine the cryo-EM structures of the paused elongation complex in complex with RNGTT, as well as the paused elongation complex in complex with RNGTT and CMTR1. Our findings show the simultaneous presence of RNGTT and the NELF complex bound to RNA polymerase II. The NELF complex exhibits two conformations, one of which shows a notable rearrangement of NELF-A/D compared to that of the paused elongation complex. Moreover, CMTR1 aligns adjacent to RNGTT on the RNA polymerase II stalk. Our structures indicate that RNGTT and CMTR1 directly bind the paused elongation complex, illuminating the mechanism by which 5'-end capping of pre-mRNA during transcriptional pausing.
External linksNat Commun / PubMed:38816438 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 4.0 Å
Structure data

37352

8w8e

EMDB-37352, PDB-8w8e:
human co-transcriptional RNA capping enzyme RNGTT
Method: EM (single particle) / Resolution: 3.9 Å

37353

8w8f

EMDB-37353, PDB-8w8f:
human co-transcriptional RNA capping enzyme RNGTT-CMTR1
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • sus scrofa (pig)
  • homo sapiens (human)
KeywordsTRANSCRIPTION / RNA polymerase II / capping / pausing / RNGTT / CMTR1

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