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TitleStructural insight into the intraflagellar transport complex IFT-A and its assembly in the anterograde IFT train.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 1506, Year 2023
Publish dateMar 17, 2023
AuthorsYuanyuan Ma / Jun He / Shaobai Li / Deqiang Yao / Chenhui Huang / Jian Wu / Ming Lei /
PubMed AbstractIntraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular transport in cilia, vital for cilia biogenesis and ...Intraflagellar transport (IFT) trains, the polymers composed of two multi-subunit complexes, IFT-A and IFT-B, carry out bidirectional intracellular transport in cilia, vital for cilia biogenesis and signaling. IFT-A plays crucial roles in the ciliary import of membrane proteins and the retrograde cargo trafficking. However, the molecular architecture of IFT-A and the assembly mechanism of the IFT-A into the IFT trains in vivo remains elusive. Here, we report the cryo-electron microscopic structures of the IFT-A complex from protozoa Tetrahymena thermophila. We find that IFT-A complexes present two distinct, elongated and folded states. Remarkably, comparison with the in situ cryo-electron tomography structure of the anterograde IFT train unveils a series of adjustments of the flexible arms in apo IFT-A when incorporated into the anterograde train. Our results provide an atomic-resolution model for the IFT-A complex and valuable insights into the assembly mechanism of anterograde IFT trains.
External linksNat Commun / PubMed:36932088 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 8.8 Å
Structure data

EMDB-34893: elongated state of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-34894: folded state of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 8.8 Å

34895

8hmc

EMDB-34895, PDB-8hmc:
base module state 1 of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 3.6 Å

34896

8hmd

EMDB-34896, PDB-8hmd:
base module state 2 of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 4.7 Å

34897

8hme

EMDB-34897, PDB-8hme:
head module state 1 of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 4.2 Å

34898

8hmf

EMDB-34898, PDB-8hmf:
head module state 2 of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-34899: head module state 3 of Tetrahymena IFT-A
Method: EM (single particle) / Resolution: 6.0 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • tetrahymena thermophila (eukaryote)
KeywordsPROTEIN TRANSPORT / intraflagellar transport complex

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