+Search query
-Structure paper
Title | Comprehensive structural analysis reveals broad-spectrum neutralizing antibodies against SARS-CoV-2 Omicron variants. |
---|---|
Journal, issue, pages | Cell Discov, Vol. 9, Issue 1, Page 37, Year 2023 |
Publish date | Apr 4, 2023 |
Authors | Xiangyang Chi / Lingyun Xia / Guanying Zhang / Ximin Chi / Bangdong Huang / Yuanyuan Zhang / Zhengshan Chen / Jin Han / Liushu Wu / Zeya Li / Hancong Sun / Ping Huang / Changming Yu / Wei Chen / Qiang Zhou / |
PubMed Abstract | The pandemic of COVID-19 caused by SARS-CoV-2 continues to spread around the world. Mutant strains of SARS-CoV-2 are constantly emerging. At present, Omicron variants have become mainstream. In this ...The pandemic of COVID-19 caused by SARS-CoV-2 continues to spread around the world. Mutant strains of SARS-CoV-2 are constantly emerging. At present, Omicron variants have become mainstream. In this work, we carried out a systematic and comprehensive analysis of the reported spike protein antibodies, counting the epitopes and genotypes of these antibodies. We further comprehensively analyzed the impact of Omicron mutations on antibody epitopes and classified these antibodies according to their binding patterns. We found that the epitopes of the H-RBD class antibodies were significantly less affected by Omicron mutations than other classes. Binding and virus neutralization experiments showed that such antibodies could effectively inhibit the immune escape of Omicron. Cryo-EM results showed that this class of antibodies utilized a conserved mechanism to neutralize SARS-CoV-2. Our results greatly help us deeply understand the impact of Omicron mutations. Meanwhile, it also provides guidance and insights for developing Omicron antibodies and vaccines. |
External links | Cell Discov / PubMed:37015915 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 4.0 Å |
Structure data | EMDB-34259, PDB-8gto: EMDB-34260: Cryo-EM map of Omicron BA.5 S protein in complex with XGv282 focused on RBD_XGv282 sub-complex EMDB-34261, PDB-8gtp: EMDB-34262: Cryo-EM map of Omicron BA.5 S protein in complex with XGv289 focused on RBD_XGv289 sub-complex EMDB-34263, PDB-8gtq: EMDB-34264: Cryo-EM map of Omicron BA.5 S protein in complex with S2L20 focused on NTD_S2L20 sub-complex |
Chemicals | ChemComp-NAG: |
Source |
|
Keywords | VIRAL PROTEIN/IMMUNE SYSTEM / SARS-CoV-2 / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex |