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TitleCryoEM structure of yeast cytoplasmic exosome complex.
Journal, issue, pagesCell Res, Vol. 26, Issue 7, Page 822-837, Year 2016
Publish dateMay 13, 2016
AuthorsJun-Jie Liu / Chu-Ya Niu / Yao Wu / Dan Tan / Yang Wang / Ming-Da Ye / Yang Liu / Wenwei Zhao / Ke Zhou / Quan-Sheng Liu / Junbiao Dai / Xuerui Yang / Meng-Qiu Dong / Niu Huang / Hong-Wei Wang /
PubMed AbstractThe eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. ...The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway. However, the interaction between Ski7 and the exosome complex has remained unclear. The transaction of RNA substrates within the exosome is also elusive. In this work, we used single-particle cryo-electron microscopy to solve the structures of the Ski7-exosome complex in RNA-free and RNA-bound forms at resolutions of 4.2 Å and 5.8 Å, respectively. These structures reveal that the N-terminal domain of Ski7 adopts a structural arrangement and interacts with the exosome in a similar fashion to the C-terminal domain of nuclear Rrp6. Further structural analysis of exosomes with RNA substrates harboring 3' overhangs of different length suggests a switch mechanism of RNA-induced exosome activation in the through-core pathway of RNA processing.
External linksCell Res / PubMed:27174052 / PubMed Central
MethodsEM (single particle)
Resolution4.2 - 13.0 Å
Structure data

3366

5g06

EMDB-3366, PDB-5g06:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-3367:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 11.5 Å

EMDB-3368:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 13.0 Å

EMDB-3369:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 5.8 Å

EMDB-3370:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-3371:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 11.0 Å

EMDB-3372:
Cryo-EM structure of yeast cytoplasmic exosome
Method: EM (single particle) / Resolution: 6.3 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsHYDROLASE / RNA DECAY / EXOSOME / RNA QUALITY CONTROL

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