Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Nasal delivery of single-domain antibody improves symptoms of SARS-CoV-2 infection in an animal model.
Journal, issue, pages	PLoS Pathog, Vol. 17, Issue 10, Page e1009542, Year 2021
Publish date	Oct 14, 2021
Authors	Kei Haga / Reiko Takai-Todaka / Yuta Matsumura / Chihong Song / Tomomi Takano / Takuto Tojo / Atsushi Nagami / Yuki Ishida / Hidekazu Masaki / Masayuki Tsuchiya / Toshiki Ebisudani / Shinya Sugimoto / Toshiro Sato / Hiroyuki Yasuda / Koichi Fukunaga / Akihito Sawada / Naoto Nemoto / Kazuyoshi Murata / Takuya Morimoto / Kazuhiko Katayama /
PubMed Abstract	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the disease COVID-19 can lead to serious symptoms, such as severe pneumonia, in the elderly and those with underlying ...The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the disease COVID-19 can lead to serious symptoms, such as severe pneumonia, in the elderly and those with underlying medical conditions. While vaccines are now available, they do not work for everyone and therapeutic drugs are still needed, particularly for treating life-threatening conditions. Here, we showed nasal delivery of a new, unmodified camelid single-domain antibody (VHH), termed K-874A, effectively inhibited SARS-CoV-2 titers in infected lungs of Syrian hamsters without causing weight loss and cytokine induction. In vitro studies demonstrated that K-874A neutralized SARS-CoV-2 in both VeroE6/TMPRSS2 and human lung-derived alveolar organoid cells. Unlike other drug candidates, K-874A blocks viral membrane fusion rather than viral attachment. Cryo-electron microscopy revealed K-874A bound between the receptor binding domain and N-terminal domain of the virus S protein. Further, infected cells treated with K-874A produced fewer virus progeny that were less infective. We propose that direct administration of K-874A to the lung could be a new treatment for preventing the reinfection of amplified virus in COVID-19 patients.
External links	PLoS Pathog / PubMed:34648602 / PubMed Central
Methods	EM (single particle)
Resolution	3.9 - 6.9 Å
Structure data	EMDB-31572: Minor cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs , focused refinement of K-874A, RBD and NTD Method: EM (single particle) / Resolution: 5.0 Å EMDB-31573: Minor cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs Method: EM (single particle) / Resolution: 4.4 Å 31574 7fg2 EMDB-31574, PDB-7fg2: Minor cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHH, composite map Method: EM (single particle) / Resolution: 4.4 Å EMDB-31575: Major cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs, focussed refinement of K-874A, RBD and NTD Method: EM (single particle) / Resolution: 5.0 Å EMDB-31576: Major cryo-EM structure of S protein trimer of SARS-CoV2 with K-874A VHHs Method: EM (single particle) / Resolution: 3.9 Å 31577 7fg3 EMDB-31577, PDB-7fg3: Major cryo-EM structure of S protein trimer of SARS-CoV2 with K-874, composite map Method: EM (single particle) / Resolution: 3.9 Å 31578 7fg7 EMDB-31578, PDB-7fg7: Cryo-EM structure of S protein trimer of SARS-CoV2 Method: EM (single particle) / Resolution: 6.9 Å
Source	Severe acute respiratory syndrome-related coronavirus severe acute respiratory syndrome coronavirus 2 homo sapiens (human)
Keywords	PROTEIN BINDING / Antibody