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TitleEB1 interacts with outwardly curved and straight regions of the microtubule lattice.
Journal, issue, pagesNat Cell Biol, Vol. 18, Issue 10, Page 1102-1108, Year 2016
Publish dateSep 12, 2016
AuthorsAudrey Guesdon / Franck Bazile / Rubén M Buey / Renu Mohan / Solange Monier / Ruddi Rodríguez García / Morgane Angevin / Claire Heichette / Ralph Wieneke / Robert Tampé / Laurence Duchesne / Anna Akhmanova / Michel O Steinmetz / Denis Chrétien /
PubMed AbstractEB1 is a microtubule plus-end tracking protein that recognizes GTP-tubulin dimers in microtubules and thus represents a unique probe to investigate the architecture of the GTP cap of growing ...EB1 is a microtubule plus-end tracking protein that recognizes GTP-tubulin dimers in microtubules and thus represents a unique probe to investigate the architecture of the GTP cap of growing microtubule ends. Here, we conjugated EB1 to gold nanoparticles (EB1-gold) and imaged by cryo-electron tomography its interaction with dynamic microtubules assembled in vitro from purified tubulin. EB1-gold forms comets at the ends of microtubules assembled in the presence of GTP, and interacts with the outer surface of curved and straight tubulin sheets as well as closed regions of the microtubule lattice. Microtubules assembled in the presence of GTP, different GTP analogues or cell extracts display similarly curved sheets at their growing ends, which gradually straighten as their protofilament number increases until they close into a tube. Together, our data provide unique structural information on the interaction of EB1 with growing microtubule ends. They further offer insights into the conformational changes that tubulin dimers undergo during microtubule assembly and the architecture of the GTP-cap region.
External linksNat Cell Biol / PubMed:27617931
MethodsEM (tomography)
Structure data

EMDB-2912:
Cryo-electron tomograms of EB1 conjugated to gold nanoparticles, in interaction with growing microtubule ends
Method: EM (tomography)

EMDB-2915:
Cryo-electron tomograms of EB1 conjugated to gold nanoparticles, in interaction with growing microtubule ends
Method: EM (tomography)

EMDB-2916:
Cryo-electron tomograms of EB1 conjugated to gold nanoparticles, in interaction with growing microtubule ends
Method: EM (tomography)

EMDB-2918:
Outwardly curved tubulin sheet at the tip of a growing microtubule
Method: EM (tomography)

EMDB-2919:
End of a microtubule assembled in the presence of GMPCPP
Method: EM (tomography)

EMDB-2920:
End of a microtubule assembled in the presence of GTP-gamma-S
Method: EM (tomography)

EMDB-2921:
End of a microtubule assembled in HeLa cytosol
Method: EM (tomography)

Source
  • Homo sapiens (human)
  • Sus scrofa (pig)

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