+Search query
-Structure paper
Title | Structures of human primosome elongation complexes. |
---|---|
Journal, issue, pages | Nat Struct Mol Biol, Vol. 30, Issue 5, Page 579-583, Year 2023 |
Publish date | Apr 17, 2023 |
Authors | Qixiang He / Andrey G Baranovskiy / Lucia M Morstadt / Alisa E Lisova / Nigar D Babayeva / Benjamin L Lusk / Ci Ji Lim / Tahir H Tahirov / |
PubMed Abstract | The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. ...The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers. |
External links | Nat Struct Mol Biol / PubMed:37069376 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.35 - 3.59 Å |
Structure data | EMDB-27256, PDB-8d96: EMDB-27258, PDB-8d9d: |
Chemicals | ChemComp-SF4: ChemComp-MG: ChemComp-DTP: ChemComp-ZN: |
Source |
|
Keywords | REPLICATION / DNA replication / human DNA polymerase alpha/primase / human primosome / elongation complex |