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| Title | Non-averaged single-molecule tertiary structures reveal RNA self-folding through individual-particle cryo-electron tomography. |
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| Journal, issue, pages | Nat Commun, Vol. 15, Issue 1, Page 9084, Year 2024 |
| Publish date | Oct 21, 2024 |
 Authors | Jianfang Liu / Ewan K S McRae / Meng Zhang / Cody Geary / Ebbe Sloth Andersen / Gang Ren /    |
| PubMed Abstract | Large-scale and continuous conformational changes in the RNA self-folding process present significant challenges for structural studies, often requiring trade-offs between resolution and ...Large-scale and continuous conformational changes in the RNA self-folding process present significant challenges for structural studies, often requiring trade-offs between resolution and observational scope. Here, we utilize individual-particle cryo-electron tomography (IPET) to examine the post-transcriptional self-folding process of designed RNA origami 6-helix bundle with a clasp helix (6HBC). By avoiding selection, classification, averaging, or chemical fixation and optimizing cryo-ET data acquisition parameters, we reconstruct 120 three-dimensional (3D) density maps from 120 individual particles at an electron dose of no more than 168 eÅ, achieving averaged resolutions ranging from 23 to 35 Å, as estimated by Fourier shell correlation (FSC) at 0.5. Each map allows us to identify distinct RNA helices and determine a unique tertiary structure. Statistical analysis of these 120 structures confirms two reported conformations and reveals a range of kinetically trapped, intermediate, and highly compacted states, demonstrating a maturation folding landscape likely driven by helix-helix compaction interactions. |
 External links | Nat Commun / PubMed:39433544 / PubMed Central |
| Methods | EM (tomography) |
| Resolution | 23.0 - 35.0 Å |
| Structure data |  EMDB-25230: Tertiary structure of an individual particle of self-folding RNA polymer (particle #121)  EMDB-25231: Tertiary structure of an individual particle of self-folding RNA polymer (particle #122)  EMDB-25232: Tertiary structure of an individual particle of self-folding RNA polymer (particle #123)  EMDB-25233: Tertiary structure of an individual particle of self-folding RNA polymer (particle #124)  EMDB-25234: Tertiary structure of an individual particle of self-folding RNA polymer (particle #125)  EMDB-25235: Tertiary structure of an individual particle of self-folding RNA polymer (particle #126)  EMDB-25236: Tertiary structure of an individual particle of self-folding RNA polymer (particle #127)  EMDB-25237: Tertiary structure of an individual particle of self-folding RNA polymer (particle #128)  EMDB-25238: Tertiary structure of an individual particle of self-folding RNA polymer (particle #129)  EMDB-25239: Tertiary structure of an individual particle of self-folding RNA polymer (particle #130)  EMDB-25240: Tertiary structure of an individual particle of self-folding RNA polymer (particle #131)  EMDB-25241: Tertiary structure of an individual particle of self-folding RNA polymer (particle #147)  EMDB-25242: Tertiary structure of an individual particle of self-folding RNA polymer (particle #148)  EMDB-25243: Tertiary structure of an individual particle of self-folding RNA polymer (particle #149)  EMDB-25244: Tertiary structure of an individual particle of self-folding RNA polymer (particle #150)  EMDB-25245: Tertiary structure of an individual particle of self-folding RNA polymer (particle #151)  EMDB-25246: Tertiary structure of an individual particle of self-folding RNA polymer (particle #152)  EMDB-25247: Tertiary structure of an individual particle of self-folding RNA polymer (particle #153)  EMDB-25248: Tertiary structure of an individual particle of self-folding RNA polymer (particle #154)  EMDB-25249: Tertiary structure of an individual particle of self-folding RNA polymer (particle #155)  EMDB-25250: Tertiary structure of an individual particle of self-folding RNA polymer (particle #132)  EMDB-25251: Tertiary structure of an individual particle of self-folding RNA polymer (particle #133)  EMDB-25252: Tertiary structure of an individual particle of self-folding RNA polymer (particle #134)  EMDB-25253: Tertiary structure of an individual particle of self-folding RNA polymer (particle #135)  EMDB-25254: Tertiary structure of an individual particle of self-folding RNA polymer (particle #136)  EMDB-25255: Tertiary structure of an individual particle of self-folding RNA polymer (particle #137)  EMDB-25256: Tertiary structure of an individual particle of self-folding RNA polymer (particle #138)  EMDB-25257: Tertiary structure of an individual particle of self-folding RNA polymer (particle #139)  EMDB-25258: Tertiary structure of an individual particle of self-folding RNA polymer (particle #140)  EMDB-25259: Tertiary structure of an individual particle of self-folding RNA polymer (particle #141)  EMDB-25260: Tertiary structure of an individual particle of self-folding RNA polymer (particle #142)  EMDB-25261: Tertiary structure of an individual particle of self-folding RNA polymer (particle #143)  EMDB-25270: Tertiary structure of an individual particle of self-folding RNA polymer (particle #144)  EMDB-25271: Tertiary structure of an individual particle of self-folding RNA polymer (particle #145)  EMDB-25272: Tertiary structure of an individual particle of self-folding RNA polymer (particle #146)  EMDB-25273: Tertiary structure of an individual particle of self-folding RNA polymer (particle #036)  EMDB-25274: Tertiary structure of an individual particle of self-folding RNA polymer (particle #037)  EMDB-25275: Tertiary structure of an individual particle of self-folding RNA polymer (particle #038)  EMDB-25276: Tertiary structure of an individual particle of self-folding RNA polymer (particle #039)  EMDB-25277: Tertiary structure of an individual particle of self-folding RNA polymer (particle #040)  EMDB-25278: Tertiary structure of an individual particle of self-folding RNA polymer (particle #041)  EMDB-25279: Tertiary structure of an individual particle of self-folding RNA polymer (particle #043)  EMDB-25280: Tertiary structure of an individual particle of self-folding RNA polymer (particle #044)  EMDB-25281: Tertiary structure of an individual particle of self-folding RNA polymer (particle #045)  EMDB-25282: Tertiary structure of an individual particle of self-folding RNA polymer (particle #047)  EMDB-25283: Tertiary structure of an individual particle of self-folding RNA polymer (particle #048)  EMDB-25284: Tertiary structure of an individual particle of self-folding RNA polymer (particle #049)  EMDB-25285: Tertiary structure of an individual particle of self-folding RNA polymer (particle #050)  EMDB-25286: Tertiary structure of an individual particle of self-folding RNA polymer (particle #051)  EMDB-25287: Tertiary structure of an individual particle of self-folding RNA polymer (particle #052)  EMDB-25288: Tertiary structure of an individual particle of self-folding RNA polymer (particle #053)  EMDB-25289: Tertiary structure of an individual particle of self-folding RNA polymer (particle #054)  EMDB-25290: Tertiary structure of an individual particle of self-folding RNA polymer (particle #055)  EMDB-25291: Tertiary structure of an individual particle of self-folding RNA polymer (particle #056)  EMDB-25292: Tertiary structure of an individual particle of self-folding RNA polymer (particle #057)  EMDB-25293: Tertiary structure of an individual particle of self-folding RNA polymer (particle #058)  EMDB-25294: Tertiary structure of an individual particle of self-folding RNA polymer (particle #059)  EMDB-25295: Tertiary structure of an individual particle of self-folding RNA polymer (particle #060)  EMDB-25296: Tertiary structure of an individual particle of self-folding RNA polymer (particle #061)  EMDB-25297: Tertiary structure of an individual particle of self-folding RNA polymer (particle #062)  EMDB-25298: Tertiary structure of an individual particle of self-folding RNA polymer (particle #063)  EMDB-25299: Tertiary structure of an individual particle of self-folding RNA polymer (particle #064)  EMDB-25300: Tertiary structure of an individual particle of self-folding RNA polymer (particle #065)  EMDB-25301: Tertiary structure of an individual particle of self-folding RNA polymer (particle #066)  EMDB-25302: Tertiary structure of an individual particle of self-folding RNA polymer (particle #067)  EMDB-25303: Tertiary structure of an individual particle of self-folding RNA polymer (particle #068)  EMDB-25304: Tertiary structure of an individual particle of self-folding RNA polymer (particle #069)  EMDB-25305: Tertiary structure of an individual particle of self-folding RNA polymer (particle #070)  EMDB-25306: Tertiary structure of an individual particle of self-folding RNA polymer (particle #071)  EMDB-25307: Tertiary structure of an individual particle of self-folding RNA polymer (particle #072)  EMDB-25308: Tertiary structure of an individual particle of self-folding RNA polymer (particle #073)  EMDB-25309: Tertiary structure of an individual particle of self-folding RNA polymer (particle #074)  EMDB-25310: Tertiary structure of an individual particle of self-folding RNA polymer (particle #075)  EMDB-25311: Tertiary structure of an individual particle of self-folding RNA polymer (particle #076)  EMDB-25312: Tertiary structure of an individual particle of self-folding RNA polymer (particle #077)  EMDB-25313: Tertiary structure of an individual particle of self-folding RNA polymer (particle #078)  EMDB-25314: Tertiary structure of an individual particle of self-folding RNA polymer (particle #079)  EMDB-25315: Tertiary structure of an individual particle of self-folding RNA polymer (particle #080)  EMDB-25316: Tertiary structure of an individual particle of self-folding RNA polymer (particle #081)  EMDB-25317: Tertiary structure of an individual particle of self-folding RNA polymer (particle #082)  EMDB-25318: Tertiary structure of an individual particle of self-folding RNA polymer (particle #084)  EMDB-25319: Tertiary structure of an individual particle of self-folding RNA polymer (particle #085)  EMDB-25320: Tertiary structure of an individual particle of self-folding RNA polymer (particle #086)  EMDB-25321: Tertiary structure of an individual particle of self-folding RNA polymer (particle #087)  EMDB-25322: Tertiary structure of an individual particle of self-folding RNA polymer (particle #088)  EMDB-25323: Tertiary structure of an individual particle of self-folding RNA polymer (particle #089)  EMDB-25324: Tertiary structure of an individual particle of self-folding RNA polymer (particle #091)  EMDB-25325: Tertiary structure of an individual particle of self-folding RNA polymer (particle #092)  EMDB-25326: Tertiary structure of an individual particle of self-folding RNA polymer (particle #093)  EMDB-25327: Tertiary structure of an individual particle of self-folding RNA polymer (particle #094)  EMDB-25328: Tertiary structure of an individual particle of self-folding RNA polymer (particle #095)  EMDB-25329: Tertiary structure of an individual particle of self-folding RNA polymer (particle #096)  EMDB-25330: Tertiary structure of an individual particle of self-folding RNA polymer (particle #097)  EMDB-25331: Tertiary structure of an individual particle of self-folding RNA polymer (particle #098)  EMDB-25332: Tertiary structure of an individual particle of self-folding RNA polymer (particle #099)  EMDB-25333: Tertiary structure of an individual particle of self-folding RNA polymer (particle #100)  EMDB-25334: Tertiary structure of an individual particle of self-folding RNA polymer (particle #101)  EMDB-25335: Tertiary structure of an individual particle of self-folding RNA polymer (particle #102)  EMDB-25336: Tertiary structure of an individual particle of self-folding RNA polymer (particle #103)  EMDB-25337: Tertiary structure of an individual particle of self-folding RNA polymer (particle #104)  EMDB-25338: Tertiary structure of an individual particle of self-folding RNA polymer (particle #105)  EMDB-25339: Tertiary structure of an individual particle of self-folding RNA polymer (particle #106)  EMDB-25340: Tertiary structure of an individual particle of self-folding RNA polymer (particle #107)  EMDB-25341: Tertiary structure of an individual particle of self-folding RNA polymer (particle #108)  EMDB-25342: Tertiary structure of an individual particle of self-folding RNA polymer (particle #109)  EMDB-25343: Tertiary structure of an individual particle of self-folding RNA polymer (particle #110)  EMDB-25344: Tertiary structure of an individual particle of self-folding RNA polymer (particle #111)  EMDB-25345: Tertiary structure of an individual particle of self-folding RNA polymer (particle #112)  EMDB-25346: Tertiary structure of an individual particle of self-folding RNA polymer (particle #113)  EMDB-25347: Tertiary structure of an individual particle of self-folding RNA polymer (particle #114)  EMDB-25348: Tertiary structure of an individual particle of self-folding RNA polymer (particle #115)  EMDB-25349: Tertiary structure of an individual particle of self-folding RNA polymer (particle #116)  EMDB-25350: Tertiary structure of an individual particle of self-folding RNA polymer (particle #117)  EMDB-25351: Tertiary structure of an individual particle of self-folding RNA polymer (particle #118)  EMDB-25352: Tertiary structure of an individual particle of self-folding RNA polymer (particle #119)  EMDB-25353: Tertiary structure of an individual particle of self-folding RNA polymer (particle #120)  EMDB-25354: Tertiary structure of an individual particle of self-folding RNA polymer (particle #090)  EMDB-25355: Tertiary structure of an individual particle of self-folding RNA polymer (particle #083)  EMDB-25356: Tertiary structure of an individual particle of self-folding RNA polymer (particle #042)  EMDB-25357: Tertiary structure of an individual particle of self-folding RNA polymer (particle #046)  EMDB-40353: Tertiary structure of an individual particle of self-folding RNA polymer (particle #001)  EMDB-40356: Tertiary structure of an individual particle of self-folding RNA polymer (particle #002)  EMDB-40357: Tertiary structure of an individual particle of self-folding RNA polymer (particle #003)  EMDB-40358: Tertiary structure of an individual particle of self-folding RNA polymer (particle #004)  EMDB-40359: Tertiary structure of an individual particle of self-folding RNA polymer (particle #005)  EMDB-40360: Tertiary structure of an individual particle of self-folding RNA polymer (particle #006)  EMDB-40361: Tertiary structure of an individual particle of self-folding RNA polymer (particle #007)  EMDB-40362: Tertiary structure of an individual particle of self-folding RNA polymer (particle #008)  EMDB-40363: Tertiary structure of an individual particle of self-folding RNA polymer (particle #009)  EMDB-40364: Tertiary structure of an individual particle of self-folding RNA polymer (particle #010)  EMDB-40365: Tertiary structure of an individual particle of self-folding RNA polymer (particle #011)  EMDB-40366: Tertiary structure of an individual particle of self-folding RNA polymer (particle #012)  EMDB-40367: Tertiary structure of an individual particle of self-folding RNA polymer (particle #013)  EMDB-40368: Tertiary structure of an individual particle of self-folding RNA polymer (particle #014)  EMDB-40369: Tertiary structure of an individual particle of self-folding RNA polymer (particle #015)  EMDB-40370: Tertiary structure of an individual particle of self-folding RNA polymer (particle #016)  EMDB-40371: Tertiary structure of an individual particle of self-folding RNA polymer (particle #017)  EMDB-40372: Tertiary structure of an individual particle of self-folding RNA polymer (particle #018)  EMDB-40373: Tertiary structure of an individual particle of self-folding RNA polymer (particle #019)  EMDB-40374: Tertiary structure of an individual particle of self-folding RNA polymer (particle #020)  EMDB-40375: Tertiary structure of an individual particle of self-folding RNA polymer (particle #021)  EMDB-40376: Tertiary structure of an individual particle of self-folding RNA polymer (particle #170)  EMDB-40377: Tertiary structure of an individual particle of self-folding RNA polymer (particle #022)  EMDB-40378: Tertiary structure of an individual particle of self-folding RNA polymer (particle #023)  EMDB-40379: Tertiary structure of an individual particle of self-folding RNA polymer (particle #024)  EMDB-40380: Tertiary structure of an individual particle of self-folding RNA polymer (particle #025)  EMDB-40381: Tertiary structure of an individual particle of self-folding RNA polymer (particle #026)  EMDB-40382: Tertiary structure of an individual particle of self-folding RNA polymer (particle #027)  EMDB-40383: Tertiary structure of an individual particle of self-folding RNA polymer (particle #028)  EMDB-40384: Tertiary structure of an individual particle of self-folding RNA polymer (particle #029)  EMDB-40385: Tertiary structure of an individual particle of self-folding RNA polymer (particle #030)  EMDB-40386: Tertiary structure of an individual particle of self-folding RNA polymer (particle #031)  EMDB-40387: Tertiary structure of an individual particle of self-folding RNA polymer (particle #032)  EMDB-40388: Tertiary structure of an individual particle of self-folding RNA polymer (particle #033)  EMDB-40389: Tertiary structure of an individual particle of self-folding RNA polymer (particle #034)  EMDB-40390: Tertiary structure of an individual particle of self-folding RNA polymer (particle #035)  EMDB-40391: Tertiary structure of an individual particle of self-folding RNA polymer (particle #156)  EMDB-40392: Tertiary structure of an individual particle of self-folding RNA polymer (particle #157)  EMDB-40393: Tertiary structure of an individual particle of self-folding RNA polymer (particle #159)  EMDB-40394: Tertiary structure of an individual particle of self-folding RNA polymer (particle #160)  EMDB-40395: Tertiary structure of an individual particle of self-folding RNA polymer (particle #161)  EMDB-40396: Tertiary structure of an individual particle of self-folding RNA polymer (particle #162)  EMDB-40397: Tertiary structure of an individual particle of self-folding RNA polymer (particle #163)  EMDB-40398: Tertiary structure of an individual particle of self-folding RNA polymer (particle #164)  EMDB-40399: Tertiary structure of an individual particle of self-folding RNA polymer (particle #165)  EMDB-40400: Tertiary structure of an individual particle of self-folding RNA polymer (particle #166)  EMDB-40401: Tertiary structure of an individual particle of self-folding RNA polymer (particle #167)  EMDB-40402: Tertiary structure of an individual particle of self-folding RNA polymer (particle #168)  EMDB-40403: Tertiary structure of an individual particle of self-folding RNA polymer (particle #169)  EMDB-40404: Tertiary structure of an individual particle of self-folding RNA polymer (particle #158) |
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