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Title | Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. |
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Journal, issue, pages | Cell, Vol. 154, Issue 6, Page 1207-1219, Year 2013 |
Publish date | Sep 12, 2013 |
Authors | Alessandro Tosi / Caroline Haas / Franz Herzog / Andrea Gilmozzi / Otto Berninghausen / Charlotte Ungewickell / Christian B Gerhold / Kristina Lakomek / Ruedi Aebersold / Roland Beckmann / Karl-Peter Hopfner / |
PubMed Abstract | INO80/SWR1 family chromatin remodelers are complexes composed of >15 subunits and molecular masses exceeding 1 MDa. Their important role in transcription and genome maintenance is exchanging the ...INO80/SWR1 family chromatin remodelers are complexes composed of >15 subunits and molecular masses exceeding 1 MDa. Their important role in transcription and genome maintenance is exchanging the histone variants H2A and H2A.Z. We report the architecture of S. cerevisiae INO80 using an integrative approach of electron microscopy, crosslinking and mass spectrometry. INO80 has an embryo-shaped head-neck-body-foot architecture and shows dynamic open and closed conformations. We can assign an Rvb1/Rvb2 heterododecamer to the head in close contact with the Ino80 Snf2 domain, Ies2, and the Arp5 module at the neck. The high-affinity nucleosome-binding Nhp10 module localizes to the body, whereas the module that contains actin, Arp4, and Arp8 maps to the foot. Structural and biochemical analyses indicate that the nucleosome is bound at the concave surface near the neck, flanked by the Rvb1/2 and Arp8 modules. Our analysis establishes a structural and functional framework for this family of large remodelers. |
External links | Cell / PubMed:24034245 |
Methods | EM (single particle) |
Resolution | 17.5 - 22.0 Å |
Structure data | EMDB-2385: EMDB-2386: |
Source |
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