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TitleCharacterization of the Core Ribosomal Binding Region for the Oxazolidone Family of Antibiotics Using Cryo-EM.
Journal, issue, pagesACS Pharmacol Transl Sci, Vol. 3, Issue 3, Page 425-432, Year 2020
Publish dateJun 12, 2020
AuthorsAlexander Wright / Kieran Deane-Alder / Edward Marschall / Rebecca Bamert / Hari Venugopal / Trevor Lithgow / David W Lupton / Matthew J Belousoff /
PubMed AbstractLinezolid and tedizolid are oxazolidinones with established clinical utility for the treatment of Gram-positive pathogens. Over time it has become apparent that even modest structural changes to the ...Linezolid and tedizolid are oxazolidinones with established clinical utility for the treatment of Gram-positive pathogens. Over time it has become apparent that even modest structural changes to the core phenyl oxazolidinone leads to drastic changes in biological activity. Consequently, the structure-activity relationship around the core oxazolidinone is constantly evolving, often reflected with new structural motifs present in nascent oxazolidinones. Herein we describe the use of cryo-electron microscopy to examine the differences in binding of several functionally different oxazolidinones in the hopes of enhanced understanding of their SAR. Tedizolid, radezolid, T145, and contezolid have been examined within the peptidyl transferase center (PTC) of the 50S ribosomal subunit from methicillin resistant . The ribosome-antibiotic complexes were resolved to a resolution of around 3 Å enabling unambiguous assignment of how each antibiotic interacts with the PTC.
External linksACS Pharmacol Transl Sci / PubMed:32566908 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.2 Å
Structure data

21872

6wqn

EMDB-21872, PDB-6wqn:
Structure of the 50S subunit of the ribosome from Methicillin Resistant Staphylococcus aureus in complex with the antibiotic, contezolid
Method: EM (single particle) / Resolution: 2.9 Å

21873

6wqq

EMDB-21873, PDB-6wqq:
Structure of the 50S subunit of the ribosome from Methicillin Resistant Staphylococcus aureus in complex with the antibiotic, radezolid
Method: EM (single particle) / Resolution: 3.1 Å

21887

6wrs

EMDB-21887, PDB-6wrs:
Structure of the 50S subunit of the ribosome from Methicillin Resistant Staphylococcus aureus in complex with the antibiotic, tedizolid
Method: EM (single particle) / Resolution: 3.2 Å

21888

6wru

EMDB-21888, PDB-6wru:
Structure of the 50S subunit of the ribosome from Methicillin Resistant Staphylococcus aureus in complex with an isomer of the tedizolid
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ZC0:
Contezolid

ChemComp-RD8:
Radezolid / antibiotic*YM

ChemComp-U7V:
Tedizolid / antibiotic*YM

ChemComp-U7Y:
Tedizolid isomer

Source
  • staphylococcus aureus (bacteria)
KeywordsRIBOSOME / antibiotic / contezolid / oxazolidinone / radezolid / tedizolid

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