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TitleSubnanometer structures of HIV-1 envelope trimers on aldrithiol-2-inactivated virus particles.
Journal, issue, pagesNat Struct Mol Biol, Vol. 27, Issue 8, Page 726-734, Year 2020
Publish dateJun 29, 2020
AuthorsZe Li / Wenwei Li / Maolin Lu / Julian Bess / Cara W Chao / Jason Gorman / Daniel S Terry / Baoshan Zhang / Tongqing Zhou / Scott C Blanchard / Peter D Kwong / Jeffrey D Lifson / Walther Mothes / Jun Liu /
PubMed AbstractThe HIV-1 envelope glycoprotein (Env) trimer, composed of gp120 and gp41 subunits, mediates viral entry into cells. Recombinant Env trimers have been studied structurally, but characterization of Env ...The HIV-1 envelope glycoprotein (Env) trimer, composed of gp120 and gp41 subunits, mediates viral entry into cells. Recombinant Env trimers have been studied structurally, but characterization of Env embedded in intact virus membranes has been limited to low resolution. Here, we deploy cryo-electron tomography and subtomogram averaging to determine the structures of Env trimers on aldrithiol-2 (AT-2)-inactivated virions in ligand-free, antibody-bound and CD4-bound forms at subnanometer resolution. Tomographic reconstructions document molecular features consistent with high-resolution structures of engineered soluble and detergent-solubilized Env trimers. One of three conformational states previously predicted by smFRET was not observed by cryo-ET, potentially owing to AT-2 inactivation. We did observe Env trimers to open in situ in response to CD4 binding, with an outward movement of gp120-variable loops and an extension of a critical gp41 helix. Overall features of Env trimer embedded in AT-2-treated virions appear well-represented by current engineered trimers.
External linksNat Struct Mol Biol / PubMed:32601441 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution9.7 - 11.5 Å
Structure data

EMDB-21411:
HIV envelope glycoprotein bound with soluble CD4 (D1-D2) and antibody 17b on AT-2 treated BaL strain virus
Method: EM (subtomogram averaging) / Resolution: 9.7 Å

EMDB-21412:
Ligand-free HIV envelope glycoprotein on AT-2 treated BaL strain virus
Method: EM (subtomogram averaging) / Resolution: 11.5 Å

EMDB-21413:
HIV envelope glycoprotein bound with antibodies 10-1074 and 3BNC117 on AT-2 treated BaL strain virus
Method: EM (subtomogram averaging) / Resolution: 9.9 Å

Source
  • Human immunodeficiency virus 1

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