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-Structure paper
Title | Structure of the human 20S U5 snRNP. |
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Journal, issue, pages | Nat Struct Mol Biol, Vol. 31, Issue 5, Page 752-756, Year 2024 |
Publish date | Mar 11, 2024 |
Authors | Sarah Schneider / Irina Brandina / Daniel Peter / Sonal Lagad / Angelique Fraudeau / Júlia Portell-Montserrat / Jonas Tholen / Jiangfeng Zhao / Wojciech P Galej / |
PubMed Abstract | The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. ...The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process. |
External links | Nat Struct Mol Biol / PubMed:38467877 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 3.2 Å |
Structure data | EMDB-18267, PDB-8q91: EMDB-19041, PDB-8rc0: |
Chemicals | ChemComp-GTP: |
Source |
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Keywords | SPLICING / snRNP / CD2BP2 / spliceosome / U5 |