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TitleStructural insights into the architecture and allostery of full-length AMP-activated protein kinase.
Journal, issue, pagesStructure, Vol. 19, Issue 4, Page 515-522, Year 2011
Publish dateApr 13, 2011
AuthorsLi Zhu / Lei Chen / Xiao-Ming Zhou / Yuan-Yuan Zhang / Yi-Jiong Zhang / Jing Zhao / Shang-Rong Ji / Jia-Wei Wu / Yi Wu /
PubMed AbstractAMP-activated protein kinase (AMPK) is a heterotrimeric complex composed of α catalytic subunit, β scaffolding subunit, and γ regulatory subunit with critical roles in maintaining cellular energy ...AMP-activated protein kinase (AMPK) is a heterotrimeric complex composed of α catalytic subunit, β scaffolding subunit, and γ regulatory subunit with critical roles in maintaining cellular energy homeostasis. However, the molecular architecture of the intact complex and the allostery associated with the adenosine binding-induced regulation of kinase activity remain unclear. Here, we determine the three-dimensional reconstruction and subunit organization of the full-length rat AMPK (α1β1γ1) through single-particle electron-microscopy. By comparing the structures of AMPK in ATP- and AMP-bound states, we are able to visualize the sequential conformational changes underlying kinase activation that transmits from the adenosine binding sites in the γ subunit to the kinase domain of the α subunit. These results not only make substantial revision to the current model of AMPK assembly, but also highlight a central role of the linker sequence of the α subunit in mediating the allostery of AMPK.
External linksStructure / PubMed:21481774
MethodsEM (single particle)
Resolution19.0 - 20.0 Å
Structure data

EMDB-1897:
Reconstruction of the 3D model of AMPK trimer in basal state
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1898:
Reconstruction of the 3D model of AMPK trimer in ATP binding state.
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1899:
Reconstruction of the 3D model of AMPK trimer in AMP binding state.
Method: EM (single particle) / Resolution: 19.0 Å

Source
  • Rattus norvegicus (Norway rat)

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