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TitleDiverse roles of the metal binding domains and transport mechanism of copper transporting P-type ATPases.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 2690, Year 2024
Publish dateMar 27, 2024
AuthorsZongxin Guo / Fredrik Orädd / Viktoria Bågenholm / Christina Grønberg / Jian Feng Ma / Peter Ott / Yong Wang / Magnus Andersson / Per Amstrup Pedersen / Kaituo Wang / Pontus Gourdon /
PubMed AbstractCopper transporting P-type (P-) ATPases are essential for cellular homeostasis. Nonetheless, the E1-E1P-E2P-E2 states mechanism of P-ATPases remains poorly understood. In particular, the role of the ...Copper transporting P-type (P-) ATPases are essential for cellular homeostasis. Nonetheless, the E1-E1P-E2P-E2 states mechanism of P-ATPases remains poorly understood. In particular, the role of the intrinsic metal binding domains (MBDs) is enigmatic. Here, four cryo-EM structures and molecular dynamics simulations of a P-ATPase are combined to reveal that in many eukaryotes the MBD immediately prior to the ATPase core, MBD, serves a structural role, remodeling the ion-uptake region. In contrast, the MBD prior to MBD, MBD, likely assists in copper delivery to the ATPase core. Invariant Tyr, Asn and Ser residues in the transmembrane domain assist in positioning sulfur-providing copper-binding amino acids, allowing for copper uptake, binding and release. As such, our findings unify previously conflicting data on the transport and regulation of P-ATPases. The results are critical for a fundamental understanding of cellular copper homeostasis and for comprehension of the molecular bases of P-disorders and ongoing clinical trials.
External linksNat Commun / PubMed:38538615 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.68 Å
Structure data

18202

8q73

EMDB-18202, PDB-8q73:
Copper-transporting ATPase HMA4 in E1 state apo
Method: EM (single particle) / Resolution: 3.58 Å

18203

8q74

EMDB-18203, PDB-8q74:
Copper-transporting ATPase HMA4 in E1 state with Cu
Method: EM (single particle) / Resolution: 3.68 Å

18204

8q75

EMDB-18204, PDB-8q75:
Copper-transporting ATPase HMA4 in E2P state with AlF
Method: EM (single particle) / Resolution: 3.2 Å

18205

8q76

EMDB-18205, PDB-8q76:
Copper-transporting ATPase HMA4 in E2P state with BeF
Method: EM (single particle) / Resolution: 3.29 Å

Chemicals

ChemComp-CU:
COPPER (II) ION

ChemComp-ALF:
TETRAFLUOROALUMINATE ION

ChemComp-MG:
Unknown entry

Source
  • oryza sativa japonica group (Japanese rice)
  • Saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSPORT PROTEIN / P-ATPase

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