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| Title | Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures Spanning the Nano- to Microscale. |
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| Journal, issue, pages | Adv Mater, Vol. 35, Issue 23, Page e2212065, Year 2023 |
| Publish date | Apr 25, 2023 |
 Authors | Bryan H Ferlez / Henning Kirst / Basil J Greber / Eva Nogales / Markus Sutter / Cheryl A Kerfeld /  |
| PubMed Abstract | Many bacteria use protein-based organelles known as bacterial microcompartments (BMCs) to organize and sequester sequential enzymatic reactions. Regardless of their specialized metabolic function, ...Many bacteria use protein-based organelles known as bacterial microcompartments (BMCs) to organize and sequester sequential enzymatic reactions. Regardless of their specialized metabolic function, all BMCs are delimited by a shell made of multiple structurally redundant, yet functionally diverse, hexameric (BMC-H), pseudohexameric/trimeric (BMC-T), or pentameric (BMC-P) shell protein paralogs. When expressed without their native cargo, shell proteins have been shown to self-assemble into 2D sheets, open-ended nanotubes, and closed shells of ≈40 nm diameter that are being developed as scaffolds and nanocontainers for applications in biotechnology. Here, by leveraging a strategy for affinity-based purification, it is demonstrated that a wide range of empty synthetic shells, many differing in end-cap structures, can be derived from a glycyl radical enzyme-associated microcompartment. The range of pleomorphic shells observed, which span ≈2 orders of magnitude in size from ≈25 nm to ≈1.8 µm, reveal the remarkable plasticity of BMC-based biomaterials. In addition, new capped nanotube and nanocone morphologies are observed that are consistent with a multicomponent geometric model in which architectural principles are shared among asymmetric carbon, viral protein, and BMC-based structures. |
 External links | Adv Mater / PubMed:36932732 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.4 - 3.6 Å |
| Structure data |  EMDB-16401: GRM3C BMC shell from R. palustris, T=4  EMDB-16402: GRM3C BMC shell from R. palustris, T=7 |
| Source |
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Rhodopseudomonas palustris BisB18 (phototrophic)