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Structure paper

TitleImpact of the yeast S0/uS2-cluster ribosomal protein rpS21/eS21 on rRNA folding and the architecture of small ribosomal subunit precursors.
Journal, issue, pagesPLoS One, Vol. 18, Issue 3, Page e0283698, Year 2023
Publish dateMar 30, 2023
AuthorsGisela Pöll / Joachim Griesenbeck / Herbert Tschochner / Philipp Milkereit /
PubMed AbstractRpS0/uS2, rpS2/uS5, and rpS21/eS21 form a cluster of ribosomal proteins (S0-cluster) at the head-body junction near the central pseudoknot of eukaryotic small ribosomal subunits (SSU). Previous work ...RpS0/uS2, rpS2/uS5, and rpS21/eS21 form a cluster of ribosomal proteins (S0-cluster) at the head-body junction near the central pseudoknot of eukaryotic small ribosomal subunits (SSU). Previous work in yeast indicated that S0-cluster assembly is required for the stabilisation and maturation of SSU precursors at specific post-nucleolar stages. Here, we analysed the role of S0-cluster formation for rRNA folding. Structures of SSU precursors isolated from yeast S0-cluster expression mutants or control strains were analysed by cryogenic electron microscopy. The obtained resolution was sufficient to detect individual 2'-O-methyl RNA modifications using an unbiased scoring approach. The data show how S0-cluster formation enables the initial recruitment of the pre-rRNA processing factor Nob1 in yeast. Furthermore, they reveal hierarchical effects on the pre-rRNA folding pathway, including the final maturation of the central pseudoknot. Based on these structural insights we discuss how formation of the S0-cluster determines at this early cytoplasmic assembly checkpoint if SSU precursors further mature or are degraded.
External linksPLoS One / PubMed:36996028 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 4.3 Å
Structure data

EMDB-16346: Enp1TAP-S21_A, consensus Refinement
Method: EM (single particle) / Resolution: 2.9 Å

16347

8c00

EMDB-16347, PDB-8c00:
Enp1TAP-S21_A population of yeast small ribosomal subunit precursors depleted of rpS21/eS21
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-16348: Enp1TAP_A, consensus refinement
Method: EM (single particle) / Resolution: 2.7 Å

16349

8c01

EMDB-16349: Enp1TAP_A population of yeast small ribosomal subunit precursors, multibody refinement
PDB-8c01: Enp1TAP_A population of yeast small ribosomal subunit precursors
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-16350: Enp1TAP_B, multibody refinement
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-16351: Enp1TAP_B, consensus refinement
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-16352: Slx9TAP-S21_A
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-16358: Enp1TAP_B, body 1
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-16359: Enp1TAP_B, body 2
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-16360: Enp1TAP_A, body 1
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-16361: Enp1TAP_A, body 2
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-16362: Enp1TAP-S21_A, body 1
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-16363: Enp1TAP-S21_A, body 2
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / ribosomal assembly state

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