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TitleATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography.
Journal, issue, pagesEMBO J, Vol. 42, Issue 12, Page e112466, Year 2023
Publish dateJun 15, 2023
AuthorsNoemi Zimmermann / Akira Noga / Jagan Mohan Obbineni / Takashi Ishikawa /
PubMed AbstractAxonemal outer dynein arm (ODA) motors generate force for ciliary beating. We analyzed three states of the ODA during the power stroke cycle using in situ cryo-electron tomography, subtomogram ...Axonemal outer dynein arm (ODA) motors generate force for ciliary beating. We analyzed three states of the ODA during the power stroke cycle using in situ cryo-electron tomography, subtomogram averaging, and classification. These states of force generation depict the prepower stroke, postpower stroke, and intermediate state conformations. Comparison of these conformations to published in vitro atomic structures of cytoplasmic dynein, ODA, and the Shulin-ODA complex revealed differences in the orientation and position of the dynein head. Our analysis shows that in the absence of ATP, all dynein linkers interact with the AAA3/AAA4 domains, indicating that interactions with the adjacent microtubule doublet B-tubule direct dynein orientation. For the prepower stroke conformation, there were changes in the tail that is anchored on the A-tubule. We built models starting with available high-resolution structures to generate a best-fitting model structure for the in situ pre- and postpower stroke ODA conformations, thereby showing that ODA in a complex with Shulin adopts a similar conformation as the active prepower stroke ODA in the axoneme.
External linksEMBO J / PubMed:37051721 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution30.3 - 39.8 Å
Structure data

16304

8bwy

EMDB-16304, PDB-8bwy:
In situ outer dynein arm from Chlamydomonas reinhardtii in a pre-power stroke state
Method: EM (subtomogram averaging) / Resolution: 38.0 Å

EMDB-16309: In situ outer dynein arm from Chlamydomonas reinhardtii in an intermediate state
Method: EM (subtomogram averaging) / Resolution: 39.8 Å

EMDB-16310: In situ outer dynein arm from Chlamydomonas reinhardtii in an intermediate
Method: EM (subtomogram averaging) / Resolution: 37.7 Å

16312

8bx8

EMDB-16312, PDB-8bx8:
In situ outer dynein arm from Chlamydomonas reinhardtii in the post-power stroke state
Method: EM (subtomogram averaging) / Resolution: 30.3 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • chlamydomonas reinhardtii (plant)
  • tetrahymena thermophila (eukaryote)
KeywordsMOTOR PROTEIN / axoneme / outer dynein arm / pre power stroke / dynein / post power stroke

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