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TitleMolecular mechanism and structure of Trigger Factor bound to the translating ribosome.
Journal, issue, pagesEMBO J, Vol. 27, Issue 11, Page 1622-1632, Year 2008
Publish dateJun 4, 2008
AuthorsFrieder Merz / Daniel Boehringer / Christiane Schaffitzel / Steffen Preissler / Anja Hoffmann / Timm Maier / Anna Rutkowska / Jasmin Lozza / Nenad Ban / Bernd Bukau / Elke Deuerling /
PubMed AbstractRibosome-associated chaperone Trigger Factor (TF) initiates folding of newly synthesized proteins in bacteria. Here, we pinpoint by site-specific crosslinking the sequence of molecular interactions ...Ribosome-associated chaperone Trigger Factor (TF) initiates folding of newly synthesized proteins in bacteria. Here, we pinpoint by site-specific crosslinking the sequence of molecular interactions of Escherichia coli TF and nascent chains during translation. Furthermore, we provide the first full-length structure of TF associated with ribosome-nascent chain complexes by using cryo-electron microscopy. In its active state, TF arches over the ribosomal exit tunnel accepting nascent chains in a protective void. The growing nascent chain initially follows a predefined path through the entire interior of TF in an unfolded conformation, and even after folding into a domain it remains accommodated inside the protective cavity of ribosome-bound TF. The adaptability to accept nascent chains of different length and folding states may explain how TF is able to assist co-translational folding of all kinds of nascent polypeptides during ongoing synthesis. Moreover, we suggest a model of how TF's chaperoning function can be coordinated with the co-translational processing and membrane targeting of nascent polypeptides by other ribosome-associated factors.
External linksEMBO J / PubMed:18497744 / PubMed Central
MethodsEM (single particle)
Resolution19.0 Å
Structure data

1499

2vrh

EMDB-1499, PDB-2vrh:
Structure of the E. coli trigger factor bound to a translating ribosome
Method: EM (single particle) / Resolution: 19.0 Å

Source
  • escherichia coli (E. coli)
KeywordsRIBOSOME / TRIGGER FACTOR / RIBOSOMAL PROTEIN / RIBONUCLEOPROTEIN / CO-TRANSLATIONAL PROTEIN FOLDING / ROTAMASE / CHAPERONE / ISOMERASE / CELL CYCLE / RNA-BINDING / RRNA-BINDING / CELL DIVISION / RIBOSOME-NASCENT CHAIN COMPLEX

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