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-Structure paper
Title | The structured organization of ' cell envelope. |
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Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 119, Issue 45, Page e2209111119, Year 2022 |
Publish date | Nov 8, 2022 |
Authors | Domenica Farci / Patrycja Haniewicz / Dario Piano / |
PubMed Abstract | Surface layers (S-layers) are highly ordered coats of proteins localized on the cell surface of many bacterial species. In these structures, one or more proteins form elementary units that self- ...Surface layers (S-layers) are highly ordered coats of proteins localized on the cell surface of many bacterial species. In these structures, one or more proteins form elementary units that self-assemble into a crystalline monolayer tiling the entire cell surface. Here, the cell envelope of the radiation-resistant bacterium was studied by cryo-electron microscopy, finding the crystalline regularity of the S-layer extended into the layers below (outer membrane, periplasm, and inner membrane). The cell envelope appears to be highly packed and resulting from a three-dimensional crystalline distribution of protein complexes organized in close continuity yet allowing a certain degree of free space. The presented results suggest how S-layers, at least in some species, are mesoscale assemblies behaving as structural and functional scaffolds essential for the entire cell envelope. |
External links | Proc Natl Acad Sci U S A / PubMed:36322746 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (electron crystallography) |
Resolution | 8.0 - 50.0 Å |
Structure data | EMDB-14095: Subtomogram average of Deinococcus radiodurans' cell envelope EMDB-14096: Symmetrized subtomogram average of Deinococcus radiodurans' cell envelope EMDB-14097: 3D map of the crystalline organisation of Deinococcus radiodurans' cell envelope |
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