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Title | Cooperative amyloid fibre binding and disassembly by the Hsp70 disaggregase. |
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Journal, issue, pages | EMBO J, Vol. 41, Issue 16, Page e110410, Year 2022 |
Publish date | Aug 16, 2022 |
Authors | Joseph George Beton / Jim Monistrol / Anne Wentink / Erin C Johnston / Anthony John Roberts / Bernd Gerhard Bukau / Bart W Hoogenboom / Helen R Saibil / |
PubMed Abstract | Although amyloid fibres are highly stable protein aggregates, a specific combination of human Hsp70 system chaperones can disassemble them, including fibres formed of α-synuclein, huntingtin, or Tau. ...Although amyloid fibres are highly stable protein aggregates, a specific combination of human Hsp70 system chaperones can disassemble them, including fibres formed of α-synuclein, huntingtin, or Tau. Disaggregation requires the ATPase activity of the constitutively expressed Hsp70 family member, Hsc70, together with the J domain protein DNAJB1 and the nucleotide exchange factor Apg2. Clustering of Hsc70 on the fibrils appears to be necessary for disassembly. Here we use atomic force microscopy to show that segments of in vitro assembled α-synuclein fibrils are first coated with chaperones and then undergo bursts of rapid, unidirectional disassembly. Cryo-electron tomography and total internal reflection fluorescence microscopy reveal fibrils with regions of densely bound chaperones, preferentially at one end of the fibre. Sub-stoichiometric amounts of Apg2 relative to Hsc70 dramatically increase recruitment of Hsc70 to the fibres, creating localised active zones that then undergo rapid disassembly at a rate of ~ 4 subunits per second. The observed unidirectional bursts of Hsc70 loading and unravelling may be explained by differences between the two ends of the polar fibre structure. |
External links | EMBO J / PubMed:35698800 / PubMed Central |
Methods | EM (tomography) |
Structure data | EMDB-13576: Alpha-synuclein amyloid fibres incubated with DNAJB1, Hsc70, Hsp110 and ATP EMDB-13577: Alpha-synuclein amyloid fibres incubated with DNAJB1, Hsc70, and ATP |
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