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Structure paper

TitleCryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
Journal, issue, pagesMol Cell, Vol. 81, Issue 23, Page 4891-44906.e8, Year 2021
Publish dateDec 2, 2021
AuthorsFrank Bürmann / Louise F H Funke / Jason W Chin / Jan Löwe /
PubMed AbstractThe ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF ...The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF activity within the replication terminus macrodomain is suppressed by the sequence-specific unloader MatP. Here, we present the complete atomic structure of MukBEF in complex with MatP and DNA as determined by electron cryomicroscopy (cryo-EM). The complex binds two distinct DNA double helices corresponding to the arms of a plectonemic loop. MatP-bound DNA threads through the MukBEF ring, while the second DNA is clamped by the kleisin MukF, MukE, and the MukB ATPase heads. Combinatorial cysteine cross-linking confirms this topology of DNA loop entrapment in vivo. Our findings illuminate how a class of near-ubiquitous DNA organizers with important roles in genome maintenance interacts with the bacterial chromosome.
External linksMol Cell / PubMed:34739874 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 13.0 Å
Structure data

12656

7nyw

EMDB-12656, PDB-7nyw:
Cryo-EM structure of the MukBEF-MatP-DNA head module
Method: EM (single particle) / Resolution: 3.1 Å

12657

7nyx

EMDB-12657, PDB-7nyx:
Cryo-EM structure of the MukBEF-MatP-DNA monomer (closed conformation)
Method: EM (single particle) / Resolution: 4.6 Å

12658

7nyy

EMDB-12658, PDB-7nyy:
Cryo-EM structure of the MukBEF monomer
Method: EM (single particle) / Resolution: 6.8 Å

12659

7nyz

EMDB-12659, PDB-7nyz:
Cryo-EM structure of the MukBEF-MatP-DNA monomer (partially open conformation)
Method: EM (single particle) / Resolution: 6.5 Å

12660

7nz0

EMDB-12660, PDB-7nz0:
Cryo-EM structure of the MukBEF-MatP-DNA monomer (open conformation)
Method: EM (single particle) / Resolution: 6.3 Å

12662

7nz2

EMDB-12662, PDB-7nz2:
Cryo-EM structure of the MukBEF-MatP-DNA tetrad
Method: EM (single particle) / Resolution: 11.0 Å

12663

7nz3

EMDB-12663, PDB-7nz3:
Cryo-EM structure of apposed MukBEF-MatP monomers on DNA
Method: EM (single particle) / Resolution: 11.0 Å

12664

7nz4

EMDB-12664, PDB-7nz4:
Cryo-EM structure of the MukBEF dimer
Method: EM (single particle) / Resolution: 13.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-PNS:
4'-PHOSPHOPANTETHEINE

Source
  • photorhabdus thracensis (bacteria)
  • escherichia coli bl21(de3) (bacteria)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / SMC-kleisin complex / ATPase

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