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TitleAnisotropic ESCRT-III architecture governs helical membrane tube formation.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 1516, Year 2020
Publish dateMay 29, 2020
AuthorsJoachim Moser von Filseck / Luca Barberi / Nathaniel Talledge / Isabel E Johnson / Adam Frost / Martin Lenz / Aurélien Roux /
PubMed AbstractESCRT-III proteins assemble into ubiquitous membrane-remodeling polymers during many cellular processes. Here we describe the structure of helical membrane tubes that are scaffolded by bundled ESCRT- ...ESCRT-III proteins assemble into ubiquitous membrane-remodeling polymers during many cellular processes. Here we describe the structure of helical membrane tubes that are scaffolded by bundled ESCRT-III filaments. Cryo-ET reveals how the shape of the helical membrane tube arises from the assembly of two distinct bundles of helical filaments that have the same helical path but bind the membrane with different interfaces. Higher-resolution cryo-EM of filaments bound to helical bicelles confirms that ESCRT-III filaments can interact with the membrane through a previously undescribed interface. Mathematical modeling demonstrates that the interface described above is key to the mechanical stability of helical membrane tubes and helps infer the rigidity of the described protein filaments. Altogether, our results suggest that the interactions between ESCRT-III filaments and the membrane could proceed through multiple interfaces, to provide assembly on membranes with various shapes, or adapt the orientation of the filaments towards the membrane during membrane remodeling.
External linksNat Commun / PubMed:32471995 / PubMed Central
MethodsEM (helical sym.) / EM (subtomogram averaging)
Resolution11.3 - 32.4 Å
Structure data

EMDB-10136:
Double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on a helical membrane bicelle
Method: EM (helical sym.) / Resolution: 14.1 Å

EMDB-10137:
Refined, asymmetrically masked double-stranded helical ESCRT-III filament formed from Snf7/Vps24/Vps2 on helical lipid bicelle
Method: EM (helical sym.) / Resolution: 11.3 Å

EMDB-10138:
Segment of helical membrane tube with longitudinal ESCRT-III filaments with different binding modes formed from Snf7/Vps24/Vps2
Method: EM (subtomogram averaging) / Resolution: 31.9 Å

EMDB-10139:
Segment of helical membrane tube with longitudinal ESCRT-III filaments in the equatorial binding mode formed from Snf7/Vps24/Vps2
Method: EM (subtomogram averaging) / Resolution: 32.4 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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