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Structure paper

TitleLarge-scale movement of eIF3 domains during translation initiation modulate start codon selection.
Journal, issue, pagesNucleic Acids Res, Vol. 49, Issue 20, Page 11491-11511, Year 2021
Publish dateNov 18, 2021
AuthorsJose L Llácer / Tanweer Hussain / Jinsheng Dong / Laura Villamayor / Yuliya Gordiyenko / Alan G Hinnebusch /
PubMed AbstractThe eukaryotic initiation factor 3 (eIF3) complex is involved in every step of translation initiation, but there is limited understanding of its molecular functions. Here, we present a single ...The eukaryotic initiation factor 3 (eIF3) complex is involved in every step of translation initiation, but there is limited understanding of its molecular functions. Here, we present a single particle electron cryomicroscopy (cryo-EM) reconstruction of yeast 48S ribosomal preinitiation complex (PIC) in an open conformation conducive to scanning, with core subunit eIF3b bound on the 40S interface near the decoding center in contact with the ternary complex eIF2·GTP·initiator tRNA. eIF3b is relocated together with eIF3i from their solvent interface locations observed in other PIC structures, with eIF3i lacking 40S contacts. Re-processing of micrographs of our previous 48S PIC in a closed state also suggests relocation of the entire eIF3b-3i-3g-3a-Cter module during the course of initiation. Genetic analysis indicates that high fidelity initiation depends on eIF3b interactions at the 40S subunit interface that promote the closed PIC conformation, or facilitate the relocation of eIF3b/eIF3i to the solvent interface, on start codon selection.
External linksNucleic Acids Res / PubMed:34648019 / PubMed Central
MethodsEM (single particle)
Resolution5.15 - 5.75 Å
Structure data

0057

6gsm

EMDB-0057: Cryo-EM structure of a partial yeast 48S preinitiation complex in its open conformation.
PDB-6gsm: Structure of a partial yeast 48S preinitiation complex in open conformation.
Method: EM (single particle) / Resolution: 5.15 Å

0058

6gsn

EMDB-0058: Cryo-EM map of a partial yeast 48S preinitiation complex in its closed conformation obtained by focused classification using masks for eIF3b-eIF3i.
PDB-6gsn: Structure of a partial yeast 48S preinitiation complex in closed conformation
Method: EM (single particle) / Resolution: 5.75 Å

Chemicals

ChemComp-7NO:
[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GCP:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-PCP, energy-carrying molecule analogue*YM

ChemComp-MET:
METHIONINE

Source
  • kluyveromyces lactis (strain atcc 8585 / cbs 2359 / dsm 70799 / nbrc 1267 / nrrl y-1140 / wm37) (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • kluyveromyces lactis (yeast)
  • saccharomyces cerevisiae s288c (yeast)
  • kluyveromyces lactis nrrl y-1140 (yeast)
KeywordsRIBOSOME / translation / initiation factors / 40S / eIF1A / eIF3 / eIF2 / tRNAi / 48S PIC / small ribosome subunit

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