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TitleStructure and mechanism of haem-dependent nitrogen-nitrogen bond formation in piperazate synthase
Journal, issue, pagesNat Catal, Year 2025
Publish dateNov 12, 2024 (structure data deposition date)
AuthorsHiggins, M.A. / Shi, X. / Soler, J. / Harland, J.B. / Parkkila, T. / Lehnert, N. / Garcia-Borras, M. / Du, Y.L. / Ryan, K.S.
External linksNat Catal / Search PubMed
MethodsX-ray diffraction
Resolution1.92 - 2.08006722536 Å
Structure data

PDB-9ebk:
Piperazate synthase (PipS) in complex with haem
Method: X-RAY DIFFRACTION / Resolution: 2.08006722536 Å

PDB-9ebm:
Piperazate synthase (PipS) in complex with haem and N5-OH-L-ornithine
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-ONH:
N~5~-hydroxy-L-ornithine

Source
  • streptomyces griseus subsp. griseus (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / N-N bond / natural product biosynthesis / heme / piperazate / piperazic acid

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