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Structure paper

TitleStructural Insights into De Novo Promoter Escape by Mycobacterium tuberculosis
Journal, issue, pagesTo Be Published
Publish dateNov 4, 2024 (structure data deposition date)
AuthorsBrewer JJ / Campbell EA / Darst SA
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.1 - 4.2 Å
Structure data

47692

9e7v

EMDB-47692, PDB-9e7v:
Off-pathway Mycobacterium tuberculosis transcription initiation promoter complex (RNA Polymerase with Sigma-A, CarD, and RbpA)
Method: EM (single particle) / Resolution: 3.4 Å

47695

9e7y

EMDB-47695, PDB-9e7y:
De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 7-mer RNA and disordered Beta' Lid element (RNA Polymerase with Sigma-A, CarD, and RbpA)
Method: EM (single particle) / Resolution: 4.2 Å

47706

9e84

EMDB-47706, PDB-9e84:
De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 6-mer RNA and disordered Sigma-A region 4 domain (RNA Polymerase with Sigma-A, CarD, and RbpA)
Method: EM (single particle) / Resolution: 3.6 Å

47707

9e85

EMDB-47707, PDB-9e85:
De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 6-mer RNA and closed Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA)
Method: EM (single particle) / Resolution: 3.1 Å

47708

9e86

EMDB-47708, PDB-9e86:
De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 5-mer RNA and open Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA)
Method: EM (single particle) / Resolution: 3.8 Å

47709

9e87

EMDB-47709, PDB-9e87:
De Novo Mycobacterium tuberculosis transcription initiation pre-RPO promoter complex with open Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA)
Method: EM (single particle) / Resolution: 3.6 Å

47710

9e88

EMDB-47710, PDB-9e88:
De novo backtracked transcription elongation complex of Mycobacterium tuberculosis RNA polymerase on a linear DNA fragment (TEC-Backtracked)
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • mycobacterium tuberculosis (bacteria)
  • synthetic construct (others)
Keywordstranscription/DNA / Promoter escape / transcription / transcription-DNA complex / TRANSCRIPTION/DNA/RNA / De novo / RNA polymerase / TRANSCRIPTION-DNA-RNA complex

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