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Structure paper

TitleStructural, computational and biochemical analysis reveal new insights in the specificity of a promiscuous GDP-Gal4E
Journal, issue, pagesTo Be Published
Publish dateDec 8, 2023 (structure data deposition date)
AuthorsAlvarez Quispe, C. / Savino, S. / Biarnes, X. / Planas, A. / Thunnissen, A.M.W.H. / Beerens, K. / Desmet, T.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.9 - 3.1 Å
Structure data

PDB-8rdg:
Crystal structure of UDP-galactose 4-epimerase from Pyrococcus horikoshii with bound NAD
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8rdh:
Crystal structure of UDP-galactose 4-epimerase from Pyrococcus horikoshii with bound NAD and GDP-L-fucose
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-8rdi:
Crystal structure of UDP-galactose 4-epimerase from Pyrococcus horikoshii containing Y145F mutation and with bound NAD and GDP-L-fucose
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-HOH:
WATER

ChemComp-GFB:
GUANOSINE-5'-DIPHOSPHATE-BETA-L-FUCOPYRANOSE

Source
  • pyrococcus horikoshii (archaea)
KeywordsISOMERASE / UDP-galactose 4-epimerase / GALE / Rossmann fold / NAD / GDP-L-fucose

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