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Structure paper

TitleStructures of Alcohol Dehydrogenases from Ralstonia and Sphingobium Spp. Reveal the Molecular Basis for Their Recognition of 'Bulky-Bulky' Ketones
Journal, issue, pagesTop. Catal., Vol. 57, Page 356-, Year 2014
Publish dateMay 10, 2013 (structure data deposition date)
AuthorsMan, H. / Kedziora, K. / Kulig, J. / Frank, A. / Lavandera, I. / Gotor-Fernandez, V. / Rother, D. / Hart, S. / Turkenburg, J.P. / Grogan, G.
External linksTop. Catal. / Search PubMed
MethodsX-ray diffraction
Resolution1.5 - 2.89 Å
Structure data

PDB-4bmn:
apo structure of short-chain alcohol dehydrogenase from Ralstonia sp. DSM 6428
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-4bms:
Short chain alcohol dehydrogenase from Ralstonia sp. DSM 6428 in complex with NADPH
Method: X-RAY DIFFRACTION / Resolution: 2.89 Å

PDB-4bmv:
Short-chain dehydrogenase from Sphingobium yanoikuyae in complex with NADPH
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-TAM:
TRIS(HYDROXYETHYL)AMINOMETHANE / pH buffer*YM

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • ralstonia sp. (bacteria)
  • sphingobium yanoikuyae (bacteria)
KeywordsOXIDOREDUCTASE / KRED / BIOCATALYST

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