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TitleX-ray crystallographic snapshots of the thioazolidine formation upon the PLP during inhibition of SufS by D-cysteine
Journal, issue, pagesto be published
Publish dateMar 31, 2022 (structure data deposition date)
AuthorsNakamura, R. / Takahashi, Y. / Fujishiro, T.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.74 - 3 Å
Structure data

PDB-7xej:
SufS with D-cysteine for 5 min
Method: X-RAY DIFFRACTION / Resolution: 1.74 Å

PDB-7xek:
SufS with D-cysteine for 30 min
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-7xel:
SufS soaked with D-penicillamine
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7xen:
SufS with L-penicillamine
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-7xeq:
NifS with D-cysteine
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-7xes:
NifS with L-penicillamine
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-9PV:
(2~{S})-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-propanoic acid

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-HOH:
WATER

ChemComp-9YX:
(2~{S},4~{S})-2-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]-1,3-thiazolidine-4-carboxylic acid

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-9ZN:
(2~{R})-3-methyl-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-butanoic acid

ChemComp-IPA:
ISOPROPYL ALCOHOL

ChemComp-CL:
Unknown entry

Source
  • bacillus subtilis subsp. subtilis str. 168 (bacteria)
  • helicobacter pylori (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / Cysteine desulfurase / Inhibitor

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