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Structure paper

TitleStructural and Mechanistic Investigations of a High-Fidelity Cas9 from Parasutterella secunda
Journal, issue, pagesAcs Catalysis, Vol. 15, Page 10109-10118, Year 2025
Publish dateNov 20, 2024 (structure data deposition date)
AuthorsShen P / Lu X / Wang C / Huang JW / Min J / Luo J / Sun Y / Chen CC / Guo RT
External linksAcs Catalysis / Search PubMed
MethodsEM (single particle)
Resolution2.7 - 2.83 Å
Structure data

62471

9ko9

EMDB-62471, PDB-9ko9:
Cryo-EM structure of PsCas9-sgRNA binary complex
Method: EM (single particle) / Resolution: 2.7 Å

62479

9kor

EMDB-62479, PDB-9kor:
Cryo-EM structure of PsCas9-sgRNA-dsDNA ternary complex
Method: EM (single particle) / Resolution: 2.8 Å

63976

9ua2

EMDB-63976, PDB-9ua2:
Cryo-EM structure of PsCas9-sgRNA-dsDNA (30 nt) ternary complex
Method: EM (single particle) / Resolution: 2.83 Å

Chemicals

ChemComp-MG:
Unknown entry

Source
  • parasutterella secunda (bacteria)
KeywordsRNA BINDING PROTEIN/RNA / CRISPR-Cas9 / RNA / binary complex / RNA BINDING PROTEIN-RNA complex / RNA BINDING PROTEIN/DNA/RNA / DNA / complex / RNA BINDING PROTEIN-DNA-RNA complex / RNA BINDING PROTEIN

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