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Structure paper

TitleN-terminal toxin signal peptides efficiently load therapeutics into a natural nano-injection system
Journal, issue, pagesBiorxiv, Year 2023
Publish dateSep 28, 2023 (structure data deposition date)
AuthorsSteiner-Rebrova EM / Ejaz RN / Kielkopf CS / Ruiz MP / Marin-Arraiza L / Hendriks IA / Nissen JN / Pozdnyakova I / Pape T / Regaiolo A ...Steiner-Rebrova EM / Ejaz RN / Kielkopf CS / Ruiz MP / Marin-Arraiza L / Hendriks IA / Nissen JN / Pozdnyakova I / Pape T / Regaiolo A / Gotz K / Heermann R / Rasmussen S / Nielsen ML / Taylor NMI
External linksBiorxiv / Search PubMed
MethodsEM (single particle)
Resolution2.14 - 3.49 Å
Structure data

EMDB-18524: Anti-feeding prophage with native Afp18 toxin
Method: EM (single particle) / Resolution: 3.49 Å

EMDB-18525: Anti-feeding prophage with Afp18DC8-Casphi2 toxin-effector chimera
Method: EM (single particle) / Resolution: 3.46 Å

EMDB-18526: Anti-feeding prophage with Afp18DC4 toxin (half of Afp18, N-terminal half)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-18527: Anti-feeding prophage with Afp18DC8-Casphi2 toxin-effector chimera
Method: EM (single particle) / Resolution: 2.16 Å

EMDB-18528: Anti-feeding prophage with Afp18DC8-Casphi2 toxin-effector chimera
Method: EM (single particle) / Resolution: 2.65 Å

EMDB-18530: Anti-feeding prophage with native Afp18 toxin
Method: EM (single particle) / Resolution: 3.31 Å

EMDB-18531: Anti-feeding prophage with Afp18DC8 (half of Afp18, N-terminal half) toxin
Method: EM (single particle) / Resolution: 3.06 Å

EMDB-18532: Anti-feeding prophage with Afp18DC8-Casphi2 toxin-effector chimera
Method: EM (single particle) / Resolution: 3.21 Å

EMDB-18551: Anti-feeding prophage without Afp18 toxin
Method: EM (single particle) / Resolution: 3.35 Å

EMDB-18552: Anti-feeding prophage with Afp18DC8-ExoU toxin-effector chimera
Method: EM (single particle) / Resolution: 2.76 Å

EMDB-18553: Anti-feeding prophage with Afp18DC8-ExoU toxin-effector chimera
Method: EM (single particle) / Resolution: 2.58 Å

EMDB-18575: Afp1-17 cap
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-18576: Afp1-16 cap
Method: EM (single particle) / Resolution: 2.85 Å

EMDB-18577: Afp1-16+ Afp18 delta C8 truncation-Casphi2 cap
Method: EM (single particle) / Resolution: 2.14 Å

EMDB-18579: Afp1-16 + Afp18 deltaC8-ExoU cap
Method: EM (single particle) / Resolution: 2.65 Å

EMDB-18580: Anti-feeding prophage with Afp18DC8-ExoU toxin-effector chimera
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • Serratia entomophila (bacteria)

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