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Structure paper

TitleCrystallographic evidence of N-carboxy-L-threonine intermediate in t6A modification of tRNA.
Journal, issue, pagesTo Be Published
Publish dateSep 28, 2024 (structure data deposition date)
AuthorsKutchaushvili, A. / Wood, E. / Luthra, A. / Hung, S.-H. / Swinehart, W. / Bayooz, S. / Scheleen, E. / Iwata-Reuyl, D. / Swairjo, M.A.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.99 - 2.05 Å
Structure data

PDB-9dsq:
Thermotoga maritima threonylcarbamoyl adenylate synthase (TsaC2) in complex with products TC-AMP and pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-9dsv:
Thermotoga maritima threonylcarbamoyl adenylate synthase (TsaC2) in complex with N-carboxy-L-threonine, magnesium and ATP
Method: X-RAY DIFFRACTION / Resolution: 2.01 Å

PDB-9dsw:
Thermotoga maritima threonylcarbamoyl adenylate synthase (TsaC2) in complex with L-threonine
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-DPO:
DIPHOSPHATE

ChemComp-TXA:
threonylcarbamoyladenylate

ChemComp-MG:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-U6A:
N-carboxy-L-threonine

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-THR:
THREONINE

Source
  • thermotoga maritima msb8 (bacteria)
  • thermotoga maritima (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / TsaC2 / TsaC / t6A / N6-threonylcarbamoyl adenosine / t6A37 / tRNA / transfer-RNA / reaction intermediate / TC-AMP

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