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TitleBiosynthesis of Spiromarmycin Reveals the Unusual Formation of Benzopyran and the Mechanistic Insight of a Promiscuous Short Chain reductase Spm14
Journal, issue, pagesTo Be Published
Publish dateOct 24, 2024 (structure data deposition date)
AuthorsJing, L.Q. / Peng, M. / Chen, Y.Y. / Ju, J.H. / Wu, D.L.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution3.1 - 3.3 Å
Structure data

PDB-9k8q:
Structure of Promiscuous Short Chain reductase Spm14
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-9k8r:
Structure of Promiscuous short chain dehydrogenase Spm14 in complex with NADPH
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • spiromastix sp. scsio f190 (fungus)
KeywordsBIOSYNTHETIC PROTEIN / Short chain dehydrogenase / coenzyme / NADPH

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