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TitleSemirationally Engineering an Efficient P450 Peroxygenase for Regio- and Enantioselective Hydroxylation of Steroids
Journal, issue, pagesAcs Catalysis, Vol. 15, Page 2977-2986, Year 2025
Publish dateSep 30, 2024 (structure data deposition date)
AuthorsFan, S. / Qin, M. / Wang, Q. / Jiang, Y. / Cong, Z.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.85 - 1.89 Å
Structure data

PDB-9js9:
Crystal structure of the CYP154C5 F92A/R114A/T248G/E282A variant from Nocardia farcinica
Method: X-RAY DIFFRACTION / Resolution: 1.89 Å

PDB-9kg5:
Crystal structure of the CYP154C5 F92A/R114A/T248D/E282A variant from Nocardia farcinica
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-TES:
TESTOSTERONE

ChemComp-HOH:
WATER

ChemComp-CD:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-GOL:
GLYCEROL

Source
  • nocardia farcinica ifm 10152 (bacteria)
KeywordsOXIDOREDUCTASE / Cytochrome P450 / Monooxygenase / Metalloprotein

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