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Structure paper

TitleGas-Sensitive Biological Crystals Processed in Pressurized Oxygen and Krypton Atmospheres: Deciphering Gas Channels in Proteins Using a Novel `Soak-and-Freeze' Methodology.
Journal, issue, pagesJ. Appl. Crystallogr., Vol. 49, Page 1478-, Year 2016
Publish dateDec 16, 2015 (structure data deposition date)
AuthorsLafumat, B. / Mueller-Dieckmann, C. / Colloc'h, N. / Prange, T. / Royant, A. / van der Linden, P. / Carpentier, P.
External linksJ. Appl. Crystallogr. / Search PubMed
MethodsX-ray diffraction
Resolution1.2 - 1.7 Å
Structure data

PDB-5frc:
Structure of urate oxidase prepared by the 'soak-and-freeze' method under 42 bar of oxygen pressure
Method: X-RAY DIFFRACTION / Resolution: 1.443 Å

PDB-5fsj:
Structure of thermolysin prepared by the 'soak-and-freeze' method under 45 bar of oxygen pressure
Method: X-RAY DIFFRACTION / Resolution: 1.201 Å

PDB-5fsp:
Structure of thermolysin prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5fss:
Structure of thermolysin prepared by the 'soak-and-freeze' method under 40 bar of krypton pressure
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5fst:
Structure of lysozyme prepared by the 'soak-and-freeze' method under 100 bar of krypton pressure
Method: X-RAY DIFFRACTION / Resolution: 1.2 Å

Chemicals

ChemComp-AZA:
8-AZAXANTHINE

ChemComp-NA:
Unknown entry

ChemComp-OXY:
OXYGEN MOLECULE

ChemComp-HOH:
WATER

ChemComp-VAL:
VALINE

ChemComp-LYS:
LYSINE

ChemComp-CA:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-KR:
KRYPTON

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

Source
  • aspergillus flavus (mold)
  • bacillus thermoproteolyticus (bacteria)
  • gallus gallus (chicken)
KeywordsOXIDOREDUCTASE / URATE OXIDASE / DIOXYGEN / PRESSURE / FLASH FREEZING / HYDROLASE / METALLOPROTEINASE / THERMOLYSINE / KRYPTON / MURAMIDASE / LYSOZYME

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