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Structure paper

Title'Catch and Release": a Variation of the Archetypal Nucleotidyl Transfer Reaction
Journal, issue, pagesAcs Catalysis, Year 2020
Publish dateJan 11, 2019 (structure data deposition date)
AuthorsSelvaraj, B. / Kocaman, S. / Trifas, M. / Serpersu, E.H. / Cuneo, M.J.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.94 - 2.5 Å
Structure data

PDB-6nmk:
Binary complex structure of the T130K mutant of ANT-4'' with Neomycin
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-6nml:
Ternary structure of the T130K mutant of ANT-4'' with Neomycin and AMPCPP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6nmm:
Ternary complex structure of the T130K mutant of ANT-4 with Neomycin, AMPCPP and Pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-NMY:
NEOMYCIN / antibiotic*YM

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / AMP-CPP, energy-carrying molecule analogue*YM

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-PPV:
PYROPHOSPHATE

Source
  • geobacillus stearothermophilus (bacteria)
Keywordstransferase/ANTIBIOTIC / Aminoglycoside nucelotidyl transferase (ANT) Aminoglycoside modifying enzymes (AGMEs) DNA polymerase / ANTIBIOTIC / transferase-ANTIBIOTIC complex

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