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TitleRevisiting strategies and their combinatorial effect for introducing peroxygenase activity in CYP102A1 (P450BM3)
Journal, issue, pagesMol Catal, Vol. 557, Page 113953-, Year 2024
Publish dateOct 27, 2023 (structure data deposition date)
AuthorsAschenbrenner, J.C. / Ebrecht, A.C. / Smit, M.S. / Opperman, D.J.
External linksMol Catal / Search PubMed
MethodsX-ray diffraction
Resolution1.8 - 1.87 Å
Structure data

PDB-8qze:
Heme-domain BM3 variant 21B3_F87V-A328F
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

PDB-8qzf:
Heme-domain BM3 mutant T268E
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-IMD:
IMIDAZOLE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • priestia megaterium (bacteria)
KeywordsOXIDOREDUCTASE / CYP / cytochrome P450 / BM3 / CYP102A1 / BM3 T268E / CYP102 T268E

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