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TitleRedesign of high-affinity nonspecific nucleases with altered sequence preference
Journal, issue, pagesTo be Published
Publish dateNov 19, 2008 (structure data deposition date)
AuthorsWang, Y.T. / Wright, J.D. / Doudeva, L.G. / Jhang, H.C. / Lim, C. / Yuan, H.S.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution2.9 Å
Structure data

PDB-3fbd:
Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsHYDROLASE/DNA / Computational redesign / Protein engineering / Protein-nucleic acid interactions / DNase / DNA hydrolysis. / Antibiotic / Antimicrobial / Bacteriocin / Endonuclease / Hydrolase / Metal-binding / Nuclease / Plasmid / Zinc / HYDROLASE-DNA COMPLEX

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