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TitleChemoenzymatic synthesis of key squalamine intermediate by engineered ketoreductase mutants from Novosphingobium aromaticivorans
Journal, issue, pagesTo Be Published
Publish dateJul 24, 2024 (structure data deposition date)
AuthorsZhang, Z. / Xu, H. / Kong, P. / Cao, J. / Li, S. / Zhang, Y. / Xu, Y. / Zhang, L.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.78 - 2 Å
Structure data

PDB-9ivn:
Crystal structure of KRED mutant-Y199A/N149L
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-9ivo:
Crystal structure of KRED mutant-Y199A/N149L
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • novosphingobium aromaticivorans (strain atcc 700278 / dsm 12444 / ccug 56034 / cip 105152 / nbrc 16084 / f199) (bacteria)
KeywordsOXIDOREDUCTASE / KRED / mutants

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