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TitleTrade-Offs between Stability and Activity of Glycosylated and Non-Glycosylated Polyester Hydrolases PHL7 and PHL7mut3
Journal, issue, pagesACS ES T Eng, Year 2025
Publish dateMar 3, 2025 (structure data deposition date)
AuthorsFohler, L. / Faschingeder, F. / Leibetseder, L. / Zhao, Z. / Useini, A. / Strater, N. / Sonnendecker, C. / Ewing, T.A. / Moers, A.P.H.A. / Werten, M.W.T. ...Fohler, L. / Faschingeder, F. / Leibetseder, L. / Zhao, Z. / Useini, A. / Strater, N. / Sonnendecker, C. / Ewing, T.A. / Moers, A.P.H.A. / Werten, M.W.T. / van Vliet, D.M. / Julsing, M.K. / Zimmermann, W. / Striedner, G.
External linksACS ES T Eng / Search PubMed
MethodsX-ray diffraction
Resolution0.89 - 1.37 Å
Structure data

PDB-9qbn:
Crystal structure of the polyester hydrolase Leipzig 7 (PHL7) mut3 variant with glycosylation by expression in Pichia pastoris (P_PHL7mut3)
Method: X-RAY DIFFRACTION / Resolution: 1.37 Å

PDB-9qde:
Crystal structure of the non-glycosylated polyester hydrolase Leipzig 7 (PHL7) mut3 variant expressed in Pichia pastoris (P_PHL7mut3_ng)
Method: X-RAY DIFFRACTION / Resolution: 1.02 Å

PDB-9qdv:
Crystal structure of the polyester hydrolase Leipzig 7 (PHL7) mut3 variant expressed in E. coli (E_PHL7mut3)
Method: X-RAY DIFFRACTION / Resolution: 0.89 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

Source
  • compost metagenome (others)
KeywordsHYDROLASE / E. coli / LCC / K. phaffii / PETase / P. pastoris

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