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TitleCombining MicroED and native mass spectrometry for structural discovery of enzyme-biosynthetic inhibitor complexes
Journal, issue, pagesTo Be Published
Publish dateFeb 15, 2025 (structure data deposition date)
AuthorsVlahakis, N.W. / Flowers, C.W. / Liu, M. / Agdanowski, M. / Johnson, S. / Summers, J.A. / Jacobs, L.M.C. / Keyser, C. / Russell, P. / Rose, S. ...Vlahakis, N.W. / Flowers, C.W. / Liu, M. / Agdanowski, M. / Johnson, S. / Summers, J.A. / Jacobs, L.M.C. / Keyser, C. / Russell, P. / Rose, S. / Orlans, J. / Adhami, N. / Chen, Y. / Sawaya, M.R. / Basu, S. / De Sanctis, D. / Wakatsuki, S. / Nelson, H.M. / Loo, J.A. / Tang, Y. / Rodriguez, J.A.
External linksSearch PubMed
MethodsX-ray diffraction / EM (electron crystallography)
Resolution1.8 - 2.5 Å
Structure data

PDB-9ncc:
Serial synchrotron X-ray diffraction structure of the apo form of papain
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-9org:
MicroED structure of apo-form CTX-M-14 beta-lactamase
Method: ELECTRON CRYSTALLOGRAPHY / Resolution: 2.5 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • carica papaya (papaya)
  • escherichia coli (E. coli)
KeywordsHYDROLASE / Enzyme / serial / protease / beta-lactamase / MicroED

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