Title | Dimeric state and novel nucleotide-binding pocket drive CpAgo-mediated DNA cleavage |
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Journal, issue, pages | To Be Published |
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Publish date | Sep 9, 2024 (structure data deposition date) |
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Authors | Liu Y / Li S / Zhang K |
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External links | Search PubMed |
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Methods | EM (single particle) |
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Resolution | 2.86 - 3.81 Å |
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Structure data | EMDB-61474, PDB-9jh7: Cryo-EM structure of CpAgo in complex with the 5'-P guide DNA. Method: EM (single particle) / Resolution: 3.4 Å EMDB-61475, PDB-9jh8: Cryo-EM structure of CpAgo_gDNA-tg_ssDNA monomeric ternary complex Method: EM (single particle) / Resolution: 3.81 Å EMDB-61476, PDB-9jh9: Cryo-EM structure of CpAgo_gDNA-tg_ssDNA dimeric ternary complex Method: EM (single particle) / Resolution: 3.42 Å EMDB-61485, PDB-9jhk: Cryo-EM structure of CpAgo_gDNA-tg_dsDNA monomeric ternary complex Method: EM (single particle) / Resolution: 3.42 Å EMDB-61486, PDB-9jhl: Cryo-EM structure of CpAgo_gDNA-tg_dsDNA dimeric ternary complex Method: EM (single particle) / Resolution: 2.86 Å EMDB-61487, PDB-9jhm: Cryo-EM structure of CpAgo_gDNA-tg_bubble_dsDNA monomeric ternary complex Method: EM (single particle) / Resolution: 3.2 Å EMDB-61488, PDB-9jhn: Cryo-EM structure of CpAgo_gDNA-tg_bubble_dsDNA dimeric ternary complex Method: EM (single particle) / Resolution: 3.0 Å |
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Chemicals | |
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Source |  clostridium perfringens (bacteria)- synthetic construct (others)
clostridium perfringenosum (bacteria)
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Keywords | DNA BINDING PROTEIN/DNA / pAgos / nucleotide-binding pocket / PAZ / cryo-EM / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / dimerization |
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