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TitleOpposite Enantioselectivity in the Bioreduction of (Z)-beta-Aryl-beta-cyanoacrylates Mediated by the Tryptophan 116 Mutants of Old Yellow Enzyme 1: Synthetic Approach to (R)- and (S)-beta-Aryl-gamma-lactams
Journal, issue, pagesAdv. Synth. Catal., Vol. 357, Page 1849-1860., Year 2015
Publish dateMar 2, 2015 (structure data deposition date)
AuthorsBrenna, E. / Crotti, M. / Gatti, F.G. / Monti, D. / Parmeggiani, F. / Powell III, R.W. / Santangelo, S. / Stewart, J.D.
External linksAdv. Synth. Catal. / Search PubMed
MethodsX-ray diffraction
Resolution1.461 - 1.498 Å
Structure data

PDB-4yil:
OYE1 W116A COMPLEXED WITH (Z)-METHYL 3-CYANO-3-(4-FLUOROPHENYL)ACRYLATE IN A NON PRODUCTIVE BINDING MODE
Method: X-RAY DIFFRACTION / Resolution: 1.461 Å

PDB-4ync:
OYE1 W116A COMPLEXED WITH (Z)-METHYL-3-CYANO-3-PHENYLACRYLATE IN A NON PRODUCTIVE BINDING MODE
Method: X-RAY DIFFRACTION / Resolution: 1.498 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-4D3:
methyl (2Z)-3-cyano-3-(4-fluorophenyl)prop-2-enoate

ChemComp-HOH:
WATER

ChemComp-4EG:
methyl (2Z)-3-cyano-3-phenylprop-2-enoate

ChemComp-CL:
Unknown entry

Source
  • saccharomyces pastorianus (lager yeast)
KeywordsOXIDOREDUCTASE / CATALYTIC ACTIVITY / FMN BINDING / OLD YELLOW ENZYME

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