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Structure paper

TitleBiophysical insights to improve affinity of antibodies to fluctuated epitope of antigen: A case of anti-Shh antibody 5E1 against Shh antigen.
Journal, issue, pagesTo Be Published
Publish dateApr 4, 2024 (structure data deposition date)
AuthorsKaneda, I. / Matsunaga, R. / Nagatoishi, S. / Senoo, A. / Caaveiro, J.M.M. / Kuroda, D. / Tsumoto, K.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.75 - 1.9 Å
Structure data

PDB-8yyz:
Crystal structure of Sonic hedgehog in complex with antibody 5E1 mutant H-R102A in the absence of metals
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8z2v:
Crystal structure of Sonic hedgehog in complex with antibody 5E1 mutant H-R102A with metals
Method: X-RAY DIFFRACTION / Resolution: 1.89 Å

PDB-8z39:
Crystal structure of Sonic hedgehog in complex with antibody 5E1 mutant L-T56A with metals
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8z3a:
Crystal structure of Sonic hedgehog in complex with antibody 5E1 without metals
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-IOD:
IODIDE ION

ChemComp-HOH:
WATER

ChemComp-ZN:
Unknown entry

ChemComp-CA:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-NO3:
NITRATE ION

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / Sonic hedgehog protein (SHH) / antibody / flexible/fluctuated epitope / affinity improvement

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