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IF

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Structure paper

Title"Catch and Release": a Variation of the Archetypal Nucleotidyl Transfer Reaction
Journal, issue, pagesAcs Catalysis, Vol. 10, Page 3548-3555, Year 2020
Publish dateMay 16, 2019 (structure data deposition date)
AuthorsSelvaraj, B. / Kocaman, S. / Trifas, M. / Serpersu, E.H. / Cuneo, M.J.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.89 - 2.3 Å
Structure data

PDB-6p01:
Apo structure of the E52D mutant of ANT-4
Method: X-RAY DIFFRACTION / Resolution: 1.89 Å

PDB-6p04:
Binary structure of the E52D mutant of ANT-4'' with Neomycin
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6p06:
Ternary structure of the E52D mutant of ANT-4 with Neomycin and AMPCPP
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6p08:
Ternary structure of the E52D mutant of ANT-4'' with Neomycin, AMP and Pyrophosphate
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NMY:
NEOMYCIN / antibiotic*YM

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / AMP-CPP, energy-carrying molecule analogue*YM

ChemComp-CA:
Unknown entry

ChemComp-PPV:
PYROPHOSPHATE

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

Source
  • bacillus sp. (bacteria)
KeywordsTRANSFERASE/ANTIBIOTIC / Aminoglycoside nucelotidyl transferase (ANT) / Aminoglycoside modifying enzymes (AGMEs) / DNA polymerase / ANTIBIOTIC / TRANSFERASE-ANTIBIOTIC complex

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