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Structure paper

TitleHydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.
Journal, issue, pagesNat. Struct. Biol., Vol. 5, Page 280-283, Year 1998
Publish dateNov 28, 1997 (structure data deposition date)
AuthorsZegers, I. / Loris, R. / Dehollander, G. / Fattah Haikal, A. / Poortmans, F. / Steyaert, J. / Wyns, L.
External linksNat. Struct. Biol. / PubMed:9546218
MethodsX-ray diffraction
Resolution1.65 - 2.2 Å
Structure data

PDB-1gsp:
RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-2gsp:
RIBONUCLEASE T1/2',3'-CGPS AND 3'-GMP, 2 DAYS
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-3gsp:
RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 4 DAYS
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4gsp:
RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-5gsp:
RIBONUCLEASE T1/3'-GMP, 9 WEEKS
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6gsp:
RIBONUCLEASE T1/3'-GMP, 15 WEEKS
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-7gsp:
RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-SGP:
GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE

ChemComp-HOH:
WATER

ChemComp-3GP:
GUANOSINE-3'-MONOPHOSPHATE

ChemComp-PO4:
PHOSPHATE ION

Source
  • aspergillus oryzae (mold)
KeywordsENDORIBONUCLEASE / HYDROLASE / RIBONUCLEASE / ENDONUCLEASE

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